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Literature summary extracted from
- Unfolding and inactivation of Abalone (Haliotis diversicolor) alkaline phosphatase during denaturation by guanidine hydrochloride (2008), Appl. Biochem. Biotechnol., 158, 323-333.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.1 | guanidine hydrochloride | inactivation of the enzyme by GuHCl (guanidine hydrochloride) is a slow, reversible reaction with fractional remaining activity. The microscopic rate constants are determined. The enzyme is protected by the substrate to a certain extent during guanidine denaturation. The changes of conformation of the enzyme in different concentrations of GuHCl are studied. The inactivation occurs before the noticeable conformational changes of the enzyme molecule as a whole can be detected, which suggests that the active site of the enzyme has more flexibility than the whole enzyme molecule | Haliotis diversicolor |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.1 | Haliotis diversicolor | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.1 | crude extract is purified using ion exchange gel DEAE-32, then by filtration chromatograph through Sephadex G-200, and ion exchange gel DEAE-32 | Haliotis diversicolor |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.1 | 4-nitrophenyl phosphate + H2O | - |
Haliotis diversicolor | 4-nitrophenol + phosphate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.3.1 | alkaline phosphatase | - |
Haliotis diversicolor |
| 3.1.3.1 | ALPase | - |
Haliotis diversicolor |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.1 | 37 | - |
assay at | Haliotis diversicolor |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.1 | 10.1 | - |
assay at | Haliotis diversicolor |
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Release 2023.1 (January 2023)
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