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Literature summary extracted from

  • Szczepanowski, R.; Krahn, I.; Bohn, N.; Puehler, A.; Schlueter, A.
    Novel macrolide resistance module carried by the IncP-1beta resistance plasmid pRSB111, isolated from a wastewater treatment plant (2007), Antimicrob. Agents Chemother., 51, 673-678.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.136 expressed in Escherichia coli, strain DH5alpha mcr containing the pBCSK vector carrying a chloramphenicol resistance gene and the pRSB111 erythromycin resistance-conferring region, recombinant expression in Pseudomonas sp. strain B13 GFP1 uncultured bacterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.136 ATP + azithromycin uncultured bacterium
-
 ADP + azithromycin 2'-O-phosphate
-
 ?
2.7.1.136 ATP + clarithromycin uncultured bacterium
-
 ADP + clarithromycin 2'-O-phosphate
-
 ?
2.7.1.136 ATP + erythromycin uncultured bacterium
-
 ADP + erythromycin-2'-O-phosphate
-
 ?
2.7.1.136 ATP + roxithromycin uncultured bacterium
-
 ADP + roxithromycin 2'-O-phosphate
-
 ?
2.7.1.136 ATP + tylosin uncultured bacterium
-
 ADP + tylosin 2'-O-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.136 uncultured bacterium Q5W3B4 macrolide resistance plasmid pRSB111, isolated from bacteria residing in the final effluents of a wastewater treatment plant
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.136 additional information
-
 accessory region on plasmid pRSB111 contains a new macrolide resistance operon, mph(E) gene encodes a macrolide phosphotransferase required for high-level macrolide resistance, resistance analyzed by determining values for minimum inhibitory concentration uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.136 ATP + azithromycin
-
 uncultured bacterium ADP + azithromycin 2'-O-phosphate
-
 ?
2.7.1.136 ATP + azithromycin recombinant expression of the pRSB111 plasmid in Pseudomonas sp. strain B13 uncultured bacterium ADP + azithromycin 2'-O-phosphate
-
 ?
2.7.1.136 ATP + clarithromycin
-
 uncultured bacterium ADP + clarithromycin 2'-O-phosphate
-
 ?
2.7.1.136 ATP + clarithromycin recombinant expression of the pRSB111 plasmid in Pseudomonas sp. strain B13 uncultured bacterium ADP + clarithromycin 2'-O-phosphate
-
 ?
2.7.1.136 ATP + erythromycin
-
 uncultured bacterium ADP + erythromycin-2'-O-phosphate
-
 ?
2.7.1.136 ATP + erythromycin recombinant expression of the pRSB111 plasmid in Pseudomonas sp. strain B13 uncultured bacterium ADP + erythromycin-2'-O-phosphate
-
 ?
2.7.1.136 ATP + roxithromycin
-
 uncultured bacterium ADP + roxithromycin 2'-O-phosphate
-
 ?
2.7.1.136 ATP + roxithromycin recombinant expression of the pRSB111 plasmid in Pseudomonas sp. strain B13 uncultured bacterium ADP + roxithromycin 2'-O-phosphate
-
 ?
2.7.1.136 ATP + tylosin
-
 uncultured bacterium ADP + tylosin 2'-O-phosphate
-
 ?
2.7.1.136 ATP + tylosin recombinant expression of the pRSB111 plasmid in Pseudomonas sp. strain B13 uncultured bacterium ADP + tylosin 2'-O-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.136 macrolide 2'-phosphotransferase
-
 uncultured bacterium