Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Arotupin, D.J.; Akinyosoye, F.A.; Onifade, A.K.
    Purification and characterization of pectin methylesterase from Aspergillus repens isolated from cultivated soil (2008), Afr. J. Biotechnol., 7, 1991-1998.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.11 EDTA
-
 Aspergillus pseudoglaucus
3.1.1.11 HgCl2 1.0 mM of HgCl2 results in approximately 50% loss of enzyme activity, while concentration of 8 mM produces complete loss of enzyme activity Aspergillus pseudoglaucus
3.1.1.11 iodoacetic acid
-
 Aspergillus pseudoglaucus
3.1.1.11 PbCl2
-
 Aspergillus pseudoglaucus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.11 Ca2+ the highest PME activity occurrs at 20 mM of Ca2+, further increase in concentration results in the decline of the enzyme activity Aspergillus pseudoglaucus
3.1.1.11 K+ the activity of the enzyme increases with increase in the concentration of K+, further increase in concentration results in the decline of the enzyme activity Aspergillus pseudoglaucus
3.1.1.11 Mg2+ highest activity at 15 mM Mg2+, further increase in concentration results in the decline of the enzyme activity Aspergillus pseudoglaucus
3.1.1.11 Na+ the activity of the enzyme increases with increase in the concentration of Na+, further increase in concentration results in the decline of the enzyme activity Aspergillus pseudoglaucus
3.1.1.11 Zn2+ highest activity at 15 mM Zn2+, further increase in concentration results in the decline of the enzyme activity Aspergillus pseudoglaucus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.11 141300
-
 gel filtration Aspergillus pseudoglaucus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.11 Aspergillus pseudoglaucus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.11 ammonium sulfate precipitation, Sephadex G-100 gel filtration, and Sephadex C-50 gel filtration Aspergillus pseudoglaucus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.11 0.82
-
 crude extract Aspergillus pseudoglaucus
3.1.1.11 6.5
-
 after 7.93fold purification Aspergillus pseudoglaucus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.11 pectin + H2O
-
 Aspergillus pseudoglaucus methanol + pectate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.11 pectin methylesterase
-
 Aspergillus pseudoglaucus
3.1.1.11 PME
-
 Aspergillus pseudoglaucus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.11 30
-
-
 Aspergillus pseudoglaucus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.11 30
-
 the optimum temperature for the activity of pectimethylesterase is 30°C, however, within 10 min of heating approximately 50% loss of enzyme activity is lost, additional 10 min of heating results in retention of only 9% enzyme activity, and further heating results in complete loss of enzyme activity Aspergillus pseudoglaucus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.11 6.5
-
-
 Aspergillus pseudoglaucus