EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.217 | into the pGEMT-Easy vector for sequencing and subcloned into pET30a for expression in Escherichia coli BL21DE3 cells | Rubrobacter xylanophilus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.4.1.217 | for the native protein a data set extending to 2.20 A resolution is collected, for the mercury derivative a data set extending to 3.05 A, and for the ternary complex with GDP and mannose a data set extending to 2.80 A | Rubrobacter xylanophilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.217 | 43000 | - |
recombinant fusion protein, determined by SDS-PAGE | Rubrobacter xylanophilus |
2.4.1.217 | 95000 | - |
determined by gel filtration, suggesting that it is dimeric in solution | Rubrobacter xylanophilus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.217 | GDP-mannose + 3-phospho-D-glycerate | Rubrobacter xylanophilus | - |
GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.217 | Rubrobacter xylanophilus | B7SY86 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.217 | native mannosyl-3-phosphoglycerate synthase is purified from Rubrobacter xylanophilus cells using a Q-Sepharose FF and a Mono-Q column, recombinant MpgS is purified by immobilized metal-affinity chromatography on a Ni-Sepharose column | Rubrobacter xylanophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.217 | GDP-mannose + 3-phospho-D-glycerate | - |
Rubrobacter xylanophilus | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.217 | mannosyl-3-phosphoglycerate synthase | - |
Rubrobacter xylanophilus |
2.4.1.217 | MPGS | - |
Rubrobacter xylanophilus |