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Literature summary extracted from

  • Kim, H.W.; Mino, K.; Ishikawa, K.
    Crystallization and preliminary X-ray analysis of endoglucanase from Pyrococcus horikoshii (2008), Acta Crystallogr. Sect. F, 64, 1169-1171.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.4 industry potent enzyme for industrial bioprocesses, including the bio-polishing of cotton products, food processing and bioethanol production Pyrococcus horikoshii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.4 X-ray diffraction analysis of crystals of the wild-type full-length EGPh is unsuccessful, sitting-drop vapour-diffusion method Pyrococcus horikoshii

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.4 additional information EGPhDELTAC5, truncated form of the enzyme shows similar enzymatic properties to the wild-type protein. EGPhDELTAC10, lacking ten residues at the C-terminus showed significantly decreased activity. Three truncated mutants (EGPhDELTAN5, EGPhDELTAC5 and EGPhDELTAN5C5) which show no change in enzymatic properties are prepared and screened for crystallization Pyrococcus horikoshii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 40000
-
 EGPhDELTAC5, SDS-PAGE Pyrococcus horikoshii
3.2.1.4 43000
-
 wild-type protein, SDS-PAGE Pyrococcus horikoshii
3.2.1.4 43970
-
 theoretically, EGPhDELTAC5, truncated form of the enzyme Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.4 cellulose + H2O Pyrococcus horikoshii
-
 cellooligosaccharide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Pyrococcus horikoshii O58925
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 cellulose + H2O
-
 Pyrococcus horikoshii cellooligosaccharide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 beta-1,4-glucanase
-
 Pyrococcus horikoshii
3.2.1.4 Cellulase
-
 Pyrococcus horikoshii
3.2.1.4 EGPh
-
 Pyrococcus horikoshii
3.2.1.4 endoglucanase
-
 Pyrococcus horikoshii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 additional information
-
 hyperthermostable Pyrococcus horikoshii