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Literature summary extracted from

  • Rintala, E.; Pitkanen, J.; Vehkomaki, M.; Penttila, M.; Ruohonen, L.
    The ORF YNL274c (GOR1) codes for glyoxylate reductase in Saccharomyces cerevisiae (2007), Yeast, 24, 129-136.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.79 ORF YNL274c, DNA and amino acid sequence determination and analysis, expression in yeast strains, overview Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.79 additional information deletion of the glyoxylate reductase-encoding gene leads to higher biomass concentration after diauxic shift, overview Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.79 glyoxylate + NADPH + H+ Saccharomyces cerevisiae
-
 glycolate + NADP+
-
 r
1.1.1.79 glyoxylate + NADPH + H+ Saccharomyces cerevisiae BY4742
-
 glycolate + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.79 Saccharomyces cerevisiae
-
 ORF YNL274c
-
1.1.1.79 Saccharomyces cerevisiae BY4742
-
 ORF YNL274c
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.79 additional information
-
 activities of wild-type and mutant strains, overview Saccharomyces cerevisiae
1.1.1.79 0.04
-
 strain BY4742, substrate glyoxylate Saccharomyces cerevisiae
1.1.1.79 13.8
-
 purified recombinant enzyme, substrate glyoxylate Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.79 2-oxoisocaproate + NADPH + H+ low activity Saccharomyces cerevisiae 2-hydroxy-4-methylpentanoate + NADP+
-
 ?
1.1.1.79 2-oxoisocaproate + NADPH + H+ low activity Saccharomyces cerevisiae BY4742 2-hydroxy-4-methylpentanoate + NADP+
-
 ?
1.1.1.79 glyoxylate + NADPH + H+
-
 Saccharomyces cerevisiae glycolate + NADP+
-
 r
1.1.1.79 glyoxylate + NADPH + H+
-
 Saccharomyces cerevisiae BY4742 glycolate + NADP+
-
 r
1.1.1.79 hydroxypyruvate + NADPH + H+ 15% activity compared to glyoxylate Saccharomyces cerevisiae D-glycerate + NADP+
-
 ?
1.1.1.79 hydroxypyruvate + NADPH + H+ 15% activity compared to glyoxylate Saccharomyces cerevisiae BY4742 D-glycerate + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.79 GOR1
-
 Saccharomyces cerevisiae
1.1.1.79 More cf. EC 1.1.1.26 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.79 30
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.79 7
-
 assay at Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.79 NADP+
-
 Saccharomyces cerevisiae
1.1.1.79 NADPH preferred cofactor, NADH gives only 4% of the activity with NADPH Saccharomyces cerevisiae