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Literature summary extracted from

  • Koenig, P.; Oreb, M.; Hoefle, A.; Kaltofen, S.; Rippe, K.; Sinning, I.; Schleiff, E.; Tews, I.
    The GTPase cycle of the chloroplast import receptors Toc33/Toc34: implications from monomeric and dimeric structures (2008), Structure, 16, 585-596.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.5.2 expression in Escherichia coli Pisum sativum
3.6.5.2 expression in Escherichia coli Arabidopsis thaliana

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.5.2 sitting-drop vapor-diffusion technique, structure of the GMPPNP-bound state and GMP-bound state of Toc33 GTPase Arabidopsis thaliana
3.6.5.2 sitting-drop vapor-diffusion technique, structure of the GMPPNP-bound state of Toc33/34 GTPase Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.5.2 GTP + H2O Pisum sativum transport of precursor proteins across chloroplast membranes involves the GTPases Toc33/34 and Toc159 at the outer chloroplast envelope GDP + phosphate
-
 ?
3.6.5.2 GTP + H2O Arabidopsis thaliana transport of precursor proteins across chloroplast membranes involves the GTPases Toc33/34 and Toc159 at the outer chloroplast envelope GDP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.5.2 Arabidopsis thaliana O23680
-
-
3.6.5.2 Pisum sativum Q41009
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.5.2
-
 Arabidopsis thaliana
3.6.5.2 the cytosolic G domain of psToc34 (lacking the C-terminal membrane anchor) is purified mainly in the GDP-bound form after recombinant protein production in Escherichia coli Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.5.2 GTP + H2O transport of precursor proteins across chloroplast membranes involves the GTPases Toc33/34 and Toc159 at the outer chloroplast envelope Pisum sativum GDP + phosphate
-
 ?
3.6.5.2 GTP + H2O transport of precursor proteins across chloroplast membranes involves the GTPases Toc33/34 and Toc159 at the outer chloroplast envelope Arabidopsis thaliana GDP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.5.2 dimer the small GTPase Toc33/34 can homodimerize. Dimerization is independent of nucleotide loading state. GTPase activation by dimerization is sparse. The dimer is not self-activating, possibly lacking an additional regulatory layer Pisum sativum
3.6.5.2 monomer
-
 Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
3.6.5.2 GTPase Toc33
-
 Arabidopsis thaliana
3.6.5.2 GTPase Toc33/34
-
 Pisum sativum