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Literature summary extracted from

  • Kim, M.; Cho, M.K.; Song, H.; Kim, D.; Park, B.; Lee, J.H.; Kang, G.B.; Kim, S.H.; Im, Y.J.; Lee, D.; Eom, S.H.
    Crystal structure of UDP-N-acetylenolpyruvylglucosamine reductase (MurB) from Thermus caldophilus (2007), Proteins, 66, 751-754.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.98 expression of MurB in Escherichia coli strain BL21(DE3) Thermus caldophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.1.98 purified recombinant MurB, hanging drop-vapor diffusion method, 0.001 ml of protein solution containing 20 mg/ml protein in 20 mM HEPES-NaOH, pH 7.5, is mixed with 0.001 ml of reservoir solution containing 100 mM MES-NaOH, pH 6.5, 18% w/v PEG 8000 and 200 mM CaCl2, equilibration against 1 ml of mother liquor, Se-Met substituted crystals are grown under the same conditions, while the substrate-complex crystals are grown in the presence of 25 mM substrate UDPGlcNAc, crystals appear within 8 h, X-ray difraction structure determination and analysis at 1.15-1.7 A resolution Thermus caldophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.98 UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH Thermus caldophilus
-
 UDP-N-acetylmuramate + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.98 Thermus caldophilus Q5SJC8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.98 recombinant MurB from Escherichia coli strain BL21(DE3) Thermus caldophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.98 UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH
-
 Thermus caldophilus UDP-N-acetylmuramate + NADP+
-
 r
1.3.1.98 UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH i.e. enolpyruvyl-UDP-N-acetylglucosamine Thermus caldophilus UDP-N-acetylmuramate + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.3.1.98 More MurB is a type II enzyme lacking Tyr loop and split betaalphabetabeta fold Thermus caldophilus

Synonyms

EC Number Synonyms Comment Organism
1.3.1.98 More a type II enzyme lacking Tyr loop and split betaalphabetabeta fold Thermus caldophilus
1.3.1.98 MurB
-
 Thermus caldophilus
1.3.1.98 UDP-N-acetylenolpyruvylglucosamine reductase
-
 Thermus caldophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.98 FAD binding structure, overview Thermus caldophilus
1.3.1.98 NADP+
-
 Thermus caldophilus
1.3.1.98 NADPH
-
 Thermus caldophilus