Literature summary extracted from

Lin, G.Z.; Lian, Y.J.; Ryu, J.H.; Sung, M.K.; Park, J.S.; Park, H.J.; Park, B.K.; Shin, J.S.; Lee, M.S.; Cheon, C.I.
Expression and purification of His-tagged flavonol synthase of Camellia sinensis from Escherichia coli (2007), Protein Expr. Purif., 55, 287-292.

Application

EC Number	Application	Comment	Organism
1.14.20.6	synthesis	production of active flavonol synthase from Camellia sinensis in Escherichia coli, with high specific activity	Camellia sinensis

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.14.20.6	expression in Escherichia coli, His-tag	Camellia sinensis

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.14.20.6	0.139	-	dihydroquercetin	pH 7.5, 30°C	Camellia sinensis

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
1.14.20.6	Iron	presence of Fe2+ in purification buffer is required to yield active enzyme	Camellia sinensis

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.20.6	Camellia sinensis	A2TJG2	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.14.20.6	from inclusion bodies of recombinant Escherichia coli, denaturation with 6 M urea. Addition of FeSO4 to purification buffers significantly improves the recovery of active enzyme	Camellia sinensis

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.14.20.6	dihydroquercetin + 2-oxoglutarate + O2	-	Camellia sinensis	quercetin + succinate + CO2 + H2O	-	?

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.14.20.6	0.16	-	dihydroquercetin	pH 7.5, 30°C	Camellia sinensis

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Release 2023.1 (January 2023)