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Literature summary extracted from

  • DeJong, R.J.; Miller, L.M.; Molina-Cruz, A.; Gupta, L.; Kumar, S.; Barillas-Mury, C.
    Reactive oxygen species detoxification by catalase is a major determinant of fecundity in the mosquito Anopheles gambiae (2007), Proc. Natl. Acad. Sci. USA, 104, 2121-2126.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.11.1.6 medicine systemic reduction in catalase activity by dsRNA-mediated knock-down significantly reduces the reproductive output of mosquito females. Mutation S2W leads to strain G3 which shows a lower specific activity and higher Km value than the wild-type Ser-isoform. Mutation S2W destabilizes the functional tetrameric form of the enzyme. Ser/Ser females have a significantly higher fecundity than Trp/Trp females Anopheles gambiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.6 expression in Escherichia coli Anopheles gambiae

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.6 additional information systemic reduction in catalase activity by dsRNA-mediated knock-down significantly reduces the reproductive output of mosquito females Anopheles gambiae
1.11.1.6 S2W natural polymorphism. Strain S carries the Ser isoform, strain G3 carries the Trp-isoform which shows a lower specific activity and higher Km value than the Ser-isoform. Mutation S2W destabilizes the functional tetrameric form of the enzyme. Ser/Ser females have a significantly higher fecundity than Trp/Trp females Anopheles gambiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.6 42
-
 H2O2 wild-type Anopheles gambiae
1.11.1.6 65.8
-
 H2O2 mutant S2W Anopheles gambiae

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.6 Anopheles gambiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.6 H2O2
-
 Anopheles gambiae O2 + H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.6 40
-
 both wild-type and mutant S2W Anopheles gambiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.6 8
-
 both wild-type and mutant S2W Anopheles gambiae