Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kim, S.M.; Kim, Y.B.; Kuzuyama, T.; Kim, S.U.
    Two copies of 4-(cytidine 5-diphospho)-2-C-methyl-D: -erythritol kinase (CMK) gene in Ginkgo biloba: molecular cloning and functional characterization (2008), Planta, 228, 941-950.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.148 light Selective increase in the transcript abundance of GbCMK2 in the radicles by induction of light. Ginkgo biloba
2.7.1.148 methyl jasmonate Selective increase in the transcript abundance of GbCMK2 in adicles. Ginkgo biloba

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.148 The two CMK sequences encoding the mature CMK proteins are amplified with their respective primer pairs and cloned to the pMW118 vector to derive the pMW-CMK1 and the pMW-CMK2 plasmids. NMW29, an Escherichia coli ychB mutant harboring the pTMV20KM plasmid. The strain is transformed with the pMW-CMK1 and the pMW-CMK2 plasmids and selected on the LB medium without mevalonate. Ginkgo biloba

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.148 chloroplast isoenzyme contains chloroplast transit peptide sequence Ginkgo biloba 9507
-
2.7.1.148 cytosol
-
 Ginkgo biloba 5829
-
2.7.1.148 nucleus
-
 Ginkgo biloba 5634
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.148 39200
-
 theoretical value, predicted Ginkgo biloba
2.7.1.148 39900
-
 theoretical value, predicted Ginkgo biloba

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.148 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol + ATP Ginkgo biloba
-
 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol + ADP
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.148 Ginkgo biloba A6XAA8
-
-
2.7.1.148 Ginkgo biloba A6XAA9
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.148 ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol catalyzes the fourth step of 2-C-methyl-D-erythritol 4-phosphate pathway Ginkgo biloba
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.148 cotyledon 5 embryos are pooled to prepare a RNA sample, used for complementation assay Ginkgo biloba
-
2.7.1.148 radicle 5 embryos are pooled to prepare a RNA sample, used for complementation assay Ginkgo biloba
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.148 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol + ATP
-
 Ginkgo biloba 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol + ADP
-
 ir

Synonyms

EC Number Synonyms Comment Organism
2.7.1.148 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase
-
 Ginkgo biloba
2.7.1.148 CDP-ME kinase
-
 Ginkgo biloba
2.7.1.148 CMK
-
 Ginkgo biloba
2.7.1.148 GbCMK1 Isoenzyme with 433 amino acids. The identity between GbCMK2 and GbCMK1 is 62.7%. Ginkgo biloba
2.7.1.148 GbCMK2 isoenzyme with 428 amino acids. The identity between GbCMK2 and GbCMK1 is 62.7%. Ginkgo biloba
2.7.1.148 YchB
-
 Ginkgo biloba

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.148 ATP
-
 Ginkgo biloba

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.148 Ginkgo biloba theoretical value, predicted
-
 5.1
2.7.1.148 Ginkgo biloba theoretical value, predicted
-
 5.2