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Literature summary extracted from

  • Ahmad, R.; Kim, M.D.; Back, K.H.; Kim, H.S.; Lee, H.S.; Kwon, S.Y.; Murata, N.; Chung, W.I.; Kwak, S.S.
    Stress-induced expression of choline oxidase in potato plant chloroplasts confers enhanced tolerance to oxidative, salt, and drought stresses (2008), Plant Cell Rep., 27, 687-698.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.17 expressed in Escherichia coli, plant expression vector pGAH/codA,codA gene and nos terminator fragments excised from pGAH/codA, ligated to stress-inducible SWPA2 promoter, fused into pCAMBIA3300 binary vector, resulting pScodA transformed into Solanum tuberosum by using Agrobacterium tumefaciens strain AGL0 Solanum tuberosum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.17 chloroplast glycine betaine production in transgenic plants Solanum tuberosum 9507
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Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.17 choline + O2 Solanum tuberosum
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 betaine aldehyde + H2O2
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 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.17 Solanum tuberosum
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 cv. Superior, wild-type and transgenic plants expressing bacterial choline oxidase (codA) gene of Arthrobacter globiformis
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.17 leaf transgenic and wild-type Solanum tuberosum
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Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.17 additional information
-
 transgenic plants under the control of the stress-inducible SWPA2 promoter generated, RT-PCR analysis under stress-inducing conditions, glycine betaine accumulation, oxidative stress tolerance, salt stress tolerance and drought stress tolerance of transgenic plants Solanum tuberosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.17 choline + O2
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 Solanum tuberosum betaine aldehyde + H2O2
-
 ?
1.1.3.17 choline + O2 oxidation of choline to glycine betaine via the intermediate betaine aldehyde, glycine betaine production in chloroplasts of transgenic plants, stress-induced expression analysis Solanum tuberosum betaine aldehyde + H2O2
-
 ?