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Literature summary extracted from

  • Mueller, G.L.; Drincovich, M.F.; Andreo, C.S.; Lara, M.V.
    Nicotiana tabacum NADP-malic enzyme: cloning, characterization and analysis of biological role (2008), Plant Cell Physiol., 49, 469-480.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.40 aspartate 2fold activation of isozyme NADP-ME1 Nicotiana tabacum
1.1.1.40 CoA slight activation of isozyme NADP-ME2 Nicotiana tabacum
1.1.1.40 additional information no activation of isozyme NADP-ME2 by aspartate Nicotiana tabacum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.40 isozyme NADP-ME2, expression in Escherichia coli Nicotiana tabacum
1.1.1.40 nadp-me1, expression in Escherichia coli Nicotiana tabacum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.40 acetyl-CoA 40% inhibition of isozyme NADP-ME1 at 2 mM; inhibition of isozyme NADP-ME2 Nicotiana tabacum
1.1.1.40 ATP 20% inhibition of isozyme NADP-ME1 at 2 mM; inhibition of isozyme NADP-ME2 Nicotiana tabacum
1.1.1.40 CoA 30% inhibition of isozyme NADP-ME1 at 2 mM Nicotiana tabacum
1.1.1.40 D-glucose 6-phosphate 40% inhibition of isozyme NADP-ME1 at 2 mM; inhibition of isozyme NADP-ME2 Nicotiana tabacum
1.1.1.40 fumarate 20% inhibition of isozyme NADP-ME1 at 2 mM; inhibition of isozyme NADP-ME2 Nicotiana tabacum
1.1.1.40 malate inhibition of isozyme NADP-ME1 at pH 7.0 Nicotiana tabacum
1.1.1.40 additional information no inhibition of isozyme NADP-ME2 by aspartate and malate Nicotiana tabacum
1.1.1.40 oxaloacetic acid 60% inhibition of isozyme NADP-ME1 at 2 mM; inhibition of isozyme NADP-ME2 Nicotiana tabacum
1.1.1.40 pyruvate 20% inhibition of isozyme NADP-ME1 at 2 mM; inhibition of isozyme NADP-ME2 Nicotiana tabacum
1.1.1.40 succinate inhibition of isozyme NADP-ME2 Nicotiana tabacum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.40 0.00171
-
 NADP+ pH 7.3, isozyme NADP-ME1 Nicotiana tabacum
1.1.1.40 0.00429
-
 NADP+ pH 7.0, isozyme NADP-ME2 Nicotiana tabacum
1.1.1.40 0.13
-
 (S)-malate pH 7.3, isozyme NADP-ME1 Nicotiana tabacum
1.1.1.40 0.69
-
 pyruvate pH 7.0, isozyme NADP-ME1 Nicotiana tabacum
1.1.1.40 1.46
-
 (S)-malate pH 7.0, isozyme NADP-ME2 Nicotiana tabacum
1.1.1.40 2.6
-
 pyruvate pH 7.0, isozyme NADP-ME2 Nicotiana tabacum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.40 chloroplast
-
 Nicotiana tabacum 9507
-
1.1.1.40 cytosol
-
 Nicotiana tabacum 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.40 63000
-
 4 * 63000, plastidic isozyme, SDS-PAGE Nicotiana tabacum
1.1.1.40 65000
-
 4 * 65000, cytosolic isozyme, SDS-PAGE Nicotiana tabacum
1.1.1.40 260000
-
 native PAGE Nicotiana tabacum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.40 (S)-malate + NADP+ Nicotiana tabacum
-
 pyruvate + CO2 + NADPH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.40 Nicotiana tabacum Q006P9 gene nadp-me2; gene nadp-me2
-
1.1.1.40 Nicotiana tabacum Q006Q0 gene nadp-me1; gene nadp-me1
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.40 flower
-
 Nicotiana tabacum
-
1.1.1.40 leaf
-
 Nicotiana tabacum
-
1.1.1.40 root
-
 Nicotiana tabacum
-
1.1.1.40 stem
-
 Nicotiana tabacum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.40 0.048
-
 enzyme extracts from flowers Nicotiana tabacum
1.1.1.40 0.069
-
 enzyme extracts from stems Nicotiana tabacum
1.1.1.40 0.072
-
 enzyme extracts from leaves Nicotiana tabacum
1.1.1.40 0.571
-
 enzyme extract from roots Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.40 (S)-malate + NADP+
-
 Nicotiana tabacum pyruvate + CO2 + NADPH
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.40 tetramer 4 * 63000, plastidic isozyme, SDS-PAGE Nicotiana tabacum
1.1.1.40 tetramer 4 * 65000, cytosolic isozyme, SDS-PAGE Nicotiana tabacum

Synonyms

EC Number Synonyms Comment Organism
1.1.1.40 L-malate:NADP oxidoreductase (oxaloacetate decarboxylating)
-
 Nicotiana tabacum
1.1.1.40 NADP-malic enzyme
-
 Nicotiana tabacum
1.1.1.40 NADP-ME
-
 Nicotiana tabacum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.40 21.6
-
 pyruvate pH 7.0, isozyme NADP-ME2 Nicotiana tabacum
1.1.1.40 154.3
-
 (S)-malate pH 7.3, isozyme NADP-ME1 Nicotiana tabacum
1.1.1.40 158.4
-
 pyruvate pH 7.0, isozyme NADP-ME1 Nicotiana tabacum
1.1.1.40 177
-
 (S)-malate pH 7.0, isozyme NADP-ME2 Nicotiana tabacum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.40 7
-
 isozyme NADP-ME1, reverse reaction Nicotiana tabacum
1.1.1.40 7
-
 isozyme NADP-ME2, forward and reverse reaction Nicotiana tabacum
1.1.1.40 7.3
-
 isozyme NADP-ME1, forward reaction Nicotiana tabacum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.40 NADP+
-
 Nicotiana tabacum
1.1.1.40 NADPH
-
 Nicotiana tabacum