Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Seitz, C.; Vitten, M.; Steinbach, P.; Hartl, S.; Hirsche, J.; Rathje, W.; Treutter, D.; Forkmann, G.
    Redirection of anthocyanin synthesis in Osteospermum hybrida by a two-enzyme manipulation strategy (2007), Phytochemistry, 68, 824-833.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.81 additional information redirection of anthocyanin synthesis in Osteospermum hybrida towards pelargonidin derivatives, which are not synthesized in wild-type plants, by expression of Gerbera hybrida dihydroflavonol 4-reductase, the mutant plants show altered flower colours due to synthesis of pelargonidin anthocyaninsssing Gerbera DFR with a construct or RNAi-mediated suppression of F3'5'H activity results in double transgenic plants accumulating predominantly pelargonidin derivatives in flowers, overview Osteospermum hybrid cultivar

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.81 dihydrokaempferol + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 Osteospermum hybrid cultivar
-
 dihydromyricetin + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
1.14.14.81 additional information Osteospermum hybrid cultivar the enzyme is involved in biosynthesis of delphinidin-derived anthocyanins which are responsible for the rose to lilac flower colours of the petals, overview ?
-
 ?
1.14.14.81 naringenin + [reduced NADPH-hemoprotein reductase] + O2 Osteospermum hybrid cultivar
-
 (2S)-eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.81 Osteospermum hybrid cultivar Q304Q4 cv. Kalanga rosy
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.81 flower
-
 Osteospermum hybrid cultivar
-
1.14.14.81 petal
-
 Osteospermum hybrid cultivar
-
1.14.14.81 ray floret F3'5'H activity in ray floret enzyme extracts of 3 developmental stages, overview Osteospermum hybrid cultivar
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.81 additional information
-
 F3'5'H activity in ray floret enzyme extracts of 3 developmental stages, overview Osteospermum hybrid cultivar

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.81 dihydrokaempferol + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
 Osteospermum hybrid cultivar dihydromyricetin + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
1.14.14.81 additional information the enzyme is involved in biosynthesis of delphinidin-derived anthocyanins which are responsible for the rose to lilac flower colours of the petals, overview Osteospermum hybrid cultivar ?
-
 ?
1.14.14.81 naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Osteospermum hybrid cultivar (2S)-eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.81 F3'5'H
-
 Osteospermum hybrid cultivar
1.14.14.81 flavonoid 3',5'-hydroxylase
-
 Osteospermum hybrid cultivar

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.81 30
-
 assay at Osteospermum hybrid cultivar

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.81 7
-
 assay at Osteospermum hybrid cultivar