EC Number | Cloned (Comment) | Organism |
---|---|---|
1.4.3.22 | expressed in Escherichia coli Rosetta cells as a recombinant thioredoxin-His6-MPO1 fusion protein, using the plasmid pJGJ389. The recombinant MPO1 protein utilizes N-methylputrescine more efficiently than other diamines, a homology-based cloning strategy | Nicotiana tabacum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.22 | additional information | - |
additional information | the kinetic properties of the MPO1 enzyme may play an important role in determining the pyridine alkaloid profiles observed in tobacco roots | Nicotiana tabacum | |
1.4.3.22 | 0.19 | - |
N-methylputrescine | Vmax: 28.8 nkat, pH and temperature not specified in the publication | Nicotiana tabacum | |
1.4.3.22 | 0.35 | - |
1,3-diaminopropane | Vmax: 11.3 nkat, pH and temperature not specified in the publication | Nicotiana tabacum | |
1.4.3.22 | 0.76 | - |
1,4-diaminobutane | Vmax: 11.1 nkat, pH and temperature not specified in the publication | Nicotiana tabacum | |
1.4.3.22 | 1.79 | - |
cadaverine | Vmax: 5.2 nkat, pH and temperature not specified in the publication | Nicotiana tabacum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.3.22 | 106000 | - |
thioredoxin-His6-MPO1 fusion protein | Nicotiana tabacum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.3.22 | Nicotiana tabacum | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.4.3.22 | hairy root culture | - |
Nicotiana tabacum | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.3.22 | 1,3-diaminopropane + H2O + O2 | - |
Nicotiana tabacum | 3-aminopropanal + NH3 + H2O2 | - |
? | |
1.4.3.22 | 1,4-diaminobutane + H2O + O2 | - |
Nicotiana tabacum | 4-aminobutanal + NH3 + H2O2 | - |
? | |
1.4.3.22 | cadaverine + H2O + O2 | - |
Nicotiana tabacum | 5-aminopentanal + NH3 + H2O2 | - |
? | |
1.4.3.22 | N-methylputrescine + H2O + O2 | - |
Nicotiana tabacum | 4-(methylamino)butanal + NH3 + H2O2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.3.22 | methylputrescine oxidase | - |
Nicotiana tabacum |
1.4.3.22 | MPO | - |
Nicotiana tabacum |
1.4.3.22 | pWGH15 | - |
Nicotiana tabacum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.4.3.22 | physiological function | the oxidative deamination is an essential step in both pyridine and tropane alkaloid biosynthesis. MPO is part of a multigene family | Nicotiana tabacum |