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Literature summary extracted from

  • Urbonavicius, J.; Jaeger, G.; Bjoerk, G.R.
    Amino acid residues of the Escherichia coli tRNA(m5U54)methyltransferase (TrmA) critical for stability, covalent binding of tRNA and enzymatic activity (2007), Nucleic Acids Res., 35, 3297-3305.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.35 the plasmids pGP100, pDM4, pKD4 and pKD46 are used for the construction of the mutants Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.35 C324A level of total TrmA-associated peptide 41%, amount of m5U in total tRNA 4%, relative to the levels found in the wild-type Escherichia coli
2.1.1.35 D299A level of total TrmA-associated peptide 63%, amount of m5U in total tRNA 1%, relative to the levels found in the wild-type Escherichia coli
2.1.1.35 E358K level of total TrmA-associated peptide 59%, amount of m5U in total tRNA 9%, relative to the levels found in the wild-type Escherichia coli
2.1.1.35 F188A level of total TrmA-associated peptide 84%, amount of m5U in total tRNA 22%, relative to the levels found in the wild-type Escherichia coli
2.1.1.35 G220D level of total TrmA-associated peptide 76%, amount of m5U in total tRNA 2%, relative to the levels found in the wild-type Escherichia coli
2.1.1.35 G360D level of total TrmA-associated peptide 53%, amount of m5U in total tRNA 1%, relative to the levels found in the wild-type Escherichia coli
2.1.1.35 Q190A level of total TrmA-associated peptide 77%, amount of m5U in total tRNA 14%, relative to the levels found in the wild-type Escherichia coli
2.1.1.35 R302A level of total TrmA-associated peptide 82%, amount of m5U in total tRNA 87%, relative to the levels found in the wild-type Escherichia coli
2.1.1.35 W132C level of total TrmA-associated peptide 102%, amount of m5U in total tRNA 34%, relative to the levels found in the wild-type Escherichia coli
2.1.1.35 W202C level of total TrmA-associated peptide 1%, amount of m5U in total tRNA 15%, relative to the levels found in the wild-type Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.35 42000
-
 native polypeptide, determined by SDS-PAGE and immunoblotting Escherichia coli
2.1.1.35 54000
-
 complex containing a piece of the 3'-end of 16S rRNA Escherichia coli
2.1.1.35 62000
-
 during the synthesis of m5U54 a covalent 62 kDa TrmA-tRNA intermediate is formed, determined by SDS-PAGE and immunoblotting Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.35 S-adenosyl-L-methionine + tRNA containing uridine at position 54 Escherichia coli
-
 S-adenosyl-L-homocysteine + tRNA containing ribothymidine at position 54
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.35 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.35 S-adenosyl-L-methionine + tRNA containing uridine at position 54
-
 Escherichia coli S-adenosyl-L-homocysteine + tRNA containing ribothymidine at position 54
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.35 TrmA
-
 Escherichia coli
2.1.1.35 tRNA(m5U54)methyltransferase
-
 Escherichia coli
2.1.1.35 tRNA(uracil-5-)methyltransferase
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.35 S-adenosyl-L-methionine
-
 Escherichia coli