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Literature summary extracted from

  • Sachdeva, S.; Reynolds, K.A.
    Mycobacterium tuberculosis beta-ketoacyl acyl carrier protein synthase III (mtFabH) assay: principles and method (2008), Methods Mol. Med., 142, 205-213.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.180 Mycobacterium tuberculosis
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Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.180 additional information
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 assay for the Mycobacterium tuberculosis FabH (mtFabH) enzyme involved in a key initiation step in the synthesis of mycolic acids, which are an integral component of the cell wall. The assay eliminates the need for the cumbersome washing steps or specialty scintillation proximity assay beads and the preparation of acyl-carrier proteins required in other assay formats. This discontinuous assay involves the reduction of radiolabled long-chain beta-ketoacyl CoA product to its dihydroxy derivative, which partitions into a nonpolar phase for quantitation, while the reduced radiolabeled substrate derivative remains in the aqueous phase Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.3.1.180 beta-ketoacyl acyl carrier protein synthase III
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 Mycobacterium tuberculosis
2.3.1.180 KASIII
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 Mycobacterium tuberculosis
2.3.1.180 mtFabH
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 Mycobacterium tuberculosis