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Literature summary extracted from

  • Normi, Y.M.; Hiraishi, T.; Taguchi, S.; Abe, H.; Sudesh, K.; Najimudin, N.; Doi, Y.
    Characterization and properties of G4X mutants of Ralstonia eutropha PHA synthase for poly(3-hydroxybutyrate) biosynthesis in Escherichia coli (2005), Macromol. Biosci., 5, 197-206.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.304 wild-type and (His)6-tagged PhaCRe, expression in Escherichia coli. Wild-type enzyme expressed in Escherichia coli shows 35% of the enzyme from Ralstonia eutropha Cupriavidus necator

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.304 F396L about 40% of the poly(3-hydroxybutyrate) content compared to wild-type Cupriavidus necator
2.3.1.304 G4A poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 14% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has higher molecular weights with that of the wild-type Cupriavidus necator
2.3.1.304 G4C poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 24% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has higher molecular weights with that of the wild-type Cupriavidus necator
2.3.1.304 G4D poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 58% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has higher molecular weights with that of the wild-type Cupriavidus necator
2.3.1.304 G4E poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 58% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has higher molecular weights with that of the wild-type Cupriavidus necator
2.3.1.304 G4F poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 45% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has higher molecular weights with that of the wild-type Cupriavidus necator
2.3.1.304 G4H poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 56% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has higher molecular weights with that of the wild-type Cupriavidus necator
2.3.1.304 G4I poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 56% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has rather similar molecular weights with that of the wild-type Cupriavidus necator
2.3.1.304 G4K poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 58% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has higher molecular weights with that of the wild-type Cupriavidus necator
2.3.1.304 G4L poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 2% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has higher molecular weights with that of the wild-type Cupriavidus necator
2.3.1.304 G4M poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 24% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has higher molecular weights with that of the wild-type Cupriavidus necator
2.3.1.304 G4N poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 57% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has higher molecular weights with that of the wild-type Cupriavidus necator
2.3.1.304 G4P poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 54% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has higher molecular weights with that of the wild-type Cupriavidus necator
2.3.1.304 G4Q poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 55% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has rather similar molecular weights with that of the wild-type Cupriavidus necator
2.3.1.304 G4R poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 54% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has higher molecular weights with that of the wild-type Cupriavidus necator
2.3.1.304 G4S poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 56% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has rather similar molecular weights with that of the wild-type Cupriavidus necator
2.3.1.304 G4T poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 56% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has rather similar molecular weights with that of the wild-type Cupriavidus necator
2.3.1.304 G4V poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 12% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has higher molecular weights with that of the wild-type Cupriavidus necator
2.3.1.304 G4W poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 13% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has higher molecular weights with that of the wild-type Cupriavidus necator
2.3.1.304 G4Y poly(3-hydroxybutyrate) content of Escherichia coli harboring mutant PhaCRe is 54% of Ralstonia eutropha wild-type value. Poly(3-hydroxybutyrate) produced by mutant has higher molecular weights with that of the wild-type Cupriavidus necator
2.3.1.304 N208D about 40% of the poly(3-hydroxybutyrate) content compared to wild-type Cupriavidus necator

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.304 0.19
-
 (R)-3-hydroxybutyryl-CoA 25°C, pH 7.0, wild-type enzyme, based on one catalytic site in a dimeric enzyme Cupriavidus necator
2.3.1.304 0.32
-
 (R)-3-hydroxybutyryl-CoA 25°C, pH 7.0, mutant enzyme G4D, based on one catalytic site in a dimeric enzyme Cupriavidus necator

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.304 66000
-
 x * 66000, (His)6-tagged wild-type and G4D mutant PhaCRe, SDS-PAGE Cupriavidus necator

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.304 Cupriavidus necator
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.304
-
 Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.304 (R)-3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n
-
 Cupriavidus necator [(R)-3-hydroxybutanoate](n+1) + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.304 ? x * 66000, (His)6-tagged wild-type and G4D mutant PhaCRe, SDS-PAGE Cupriavidus necator

Synonyms

EC Number Synonyms Comment Organism
2.3.1.304 PhaCRe
-
 Cupriavidus necator
2.3.1.304 type I polyhydroxyalkanoate synthase
-
 Cupriavidus necator

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.304 26
-
 (R)-3-hydroxybutyryl-CoA 25°C, pH 7.0, mutant enzyme G4D, based on one catalytic site in a dimeric enzyme Cupriavidus necator
2.3.1.304 44
-
 (R)-3-hydroxybutyryl-CoA 25°C, pH 7.0, wild-type enzyme, based on one catalytic site in a dimeric enzyme Cupriavidus necator