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Literature summary extracted from

  • Cardinali, A.; Sergio, L.; Di Venere, D.; Linsalata, V.; Fortunato, D.; Conti, A.; Lattanzio, V.
    Purification and characterization of a cationic peroxidase from artichoke leaves (2007), J. Sci. Food Agric., 87, 1417-1423.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.7 51000
-
 SDS-PAGE Cynara cardunculus

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.7 Cynara cardunculus P84714 Cynara cardunculus scolymus
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.11.1.7 glycoprotein
-
 Cynara cardunculus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.7 ammonium sulfate precipitation, Sephadex G-25 gel filtration, Red Sepharose CL-6B affinity chromatography, HyperDQ high-performance liquid chromatography, and isoelectrofocusing Cynara cardunculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.7 leaf
-
 Cynara cardunculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.7 additional information
-
 the crude extract has a specific activity of 10 units/mg, the 43fold purified enzyme has a specific activity of 86.4 units/mg, one unit of peroxidase activity is defined as the absorbance change at 470 nm per minute using guaiacol assay Cynara cardunculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.7 guaiacol + H2O2
-
 Cynara cardunculus tetraguaiacol + H2O
-
 ?
1.11.1.7 additional information shows no detectable activity with ascorbic acid or veratryl alcohol Cynara cardunculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.7 ALSP
-
 Cynara cardunculus
1.11.1.7 cationic peroxidase
-
 Cynara cardunculus
1.11.1.7 hydrogen donor oxidoreductase
-
 Cynara cardunculus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.7 5 85 the enzyme shows high stability between 5 and 65°C, but is inactivated after drastic heat treatment (10 min at 85°C) Cynara cardunculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.7 5.5 6
-
 Cynara cardunculus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.7 Cynara cardunculus isoelectric focusing
-
 9