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Literature summary extracted from

  • Held, M.; Pepper, A.N.; Bozdarov, J.; Smith, M.D.; Emery, R.J.; Guinel, F.C.
    The pea nodulation mutant R50 (sym16) displays altered activity and expression profiles for cytokinin dehydrogenase (2008), J. Plant Growth Regul., 27, 170-180.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.99.12 57966
-
 x * 57966, calculated from the deduced amino acid sequence Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.99.12 additional information Pisum sativum irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.99.12 Pisum sativum A0MQ40 PsCKX1; L. cv. Sparkle, wild type and R50 carrying a recessive sym16 which is a pleiotropic mutant of pea which develops few, pale nodules and has pale young leaves, R50 has reduced CKX activity and increased cytokinin level compared with wild type, sequencing of DNA from both strains did not show any obvious mutations within the gene
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.99.12 flower
-
 Pisum sativum
-
1.5.99.12 root highest activity in root nodule, very low enzyme level in R50 mutant compared with wild type Pisum sativum
-
1.5.99.12 shoot
-
 Pisum sativum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.99.12 additional information irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde Pisum sativum ?
-
 ?
1.5.99.12 N6-(2-isopentenyl)adenine + 2,6-dichlorophenol indophenol
-
 Pisum sativum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.99.12 ? x * 57966, calculated from the deduced amino acid sequence Pisum sativum

Synonyms

EC Number Synonyms Comment Organism
1.5.99.12 CKX
-
 Pisum sativum
1.5.99.12 PsCKX1
-
 Pisum sativum
1.5.99.12 PsCKX2
-
 Pisum sativum

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.99.12 FAD
-
 Pisum sativum