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Literature summary extracted from

  • Nishimura, S.; Nagano, S.; A Crai, C.; Yokochi, N.; Yoshikane, Y.; Ge, F.; Yagi, T.
    Determination of individual vitamin B(6) compounds based on enzymatic conversion to 4-pyridoxolactone (2008), J. Nutr. Sci. Vitaminol., 54, 18-24.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.107 analysis the enzyme is useful in determination of vitamin B6 contents, method development, overview Saccharomyces cerevisiae
2.6.1.30 analysis determination method for individual natural vitamin B6 compounds. Compounds are specifically converted into 4-pyridoxolactone by pyridoxal 4-dehydrogenase and coupling reactions involving pyridoxine 4-oxidase, pyridoxal 4-dehydrogenase, or pyridoxamine-pyruvate aminotransferase and pyridoxal 4-dehydrogenase. Application of method for analysis of food samples Mesorhizobium loti

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.107 pyridoxal + NAD+ Saccharomyces cerevisiae specific conversion of vitamin B6 4-pyridoxolactone + NADH + H+
-
 ir
1.1.3.12 pyridoxine + O2 Mesorhizobium loti
-
 pyridoxal + H2O2
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.107 Saccharomyces cerevisiae
-
-
-
1.1.3.12 Mesorhizobium loti
-
-
-
2.6.1.30 Mesorhizobium loti Q988B8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.107 pyridoxal + NAD+ specific conversion of vitamin B6 Saccharomyces cerevisiae 4-pyridoxolactone + NADH + H+
-
 ir
1.1.1.107 pyridoxal + NAD+ development and evaluation of a method to determine vitamin B6, pyridoxal, involving the enzyme, overview Saccharomyces cerevisiae 4-pyridoxolactone + NADH + H+
-
 ir
1.1.3.12 pyridoxine + O2
-
 Mesorhizobium loti pyridoxal + H2O2
-
 ir
1.1.3.12 pyridoxine + O2 development of an fluorescence assay method for determination by HPLC of vitamin B6 compounds, e.g. pyridoxine and pyridoxal, coupling reaction of recombinant pyridoxine 4-oxidase and recombinant pyridoxal 4-dehydrogenase, overview Mesorhizobium loti pyridoxal + H2O2
-
 ir

Synonyms

EC Number Synonyms Comment Organism
1.1.1.107 PLDH
-
 Saccharomyces cerevisiae
1.1.3.12 pyridoxine-4-oxidase
-
 Mesorhizobium loti

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.107 30
-
 assay at Saccharomyces cerevisiae
1.1.3.12 30
-
 assay at Mesorhizobium loti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.107 additional information
-
 inactivation at low pH Saccharomyces cerevisiae
1.1.1.107 8
-
 assay at Saccharomyces cerevisiae
1.1.3.12 8
-
 assay at Mesorhizobium loti

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.107 NAD+
-
 Saccharomyces cerevisiae