EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.1.5 | expressed as a hexa-histidine-tagged fusion in Escherichia coli BL21 Star (DE3) | Paraburkholderia xenovorans LB400 |
1.2.1.77 | expressed in Escherichia coli BL21 Star (DE3) cells | Paraburkholderia xenovorans |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.2.1.5 | ALDHC in complex with NADPH bound in the cofactor binding pocket and an ordered fragment of a polyethylene glycol molecule bound in the substrate tunnel, to 1.6 A resolution. Belongs to space group P1. The ALDHC monomer comprises three distinct domains, an N-terminal cofactor (NAD/P+)-binding domain, a catalytic domain, and an oligomerization domain | Paraburkholderia xenovorans LB400 |
1.2.1.77 | sitting drop vapour diffusion method, at 18°C in 29% PEG 3350K and 100 mM bis-Tris (pH 6.0), 1.6 A crystal structure of ALDHC in complex with NADPH bound in the cofactor-binding pocket and an ordered fragment of a polyethylene glycol molecule bound in the substrate tunnel | Paraburkholderia xenovorans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.5 | 0.04 | - |
NADP+ | - |
Paraburkholderia xenovorans LB400 | |
1.2.1.5 | 0.042 | - |
Heptanal | - |
Paraburkholderia xenovorans LB400 | |
1.2.1.5 | 0.3 | - |
Valeraldehyde | - |
Paraburkholderia xenovorans LB400 | |
1.2.1.5 | 0.501 | - |
NAD+ | - |
Paraburkholderia xenovorans LB400 | |
1.2.1.5 | 1.21 | - |
propionaldehyde | - |
Paraburkholderia xenovorans LB400 | |
1.2.1.5 | 1.66 | - |
Isovaleraldehyde | - |
Paraburkholderia xenovorans LB400 | |
1.2.1.5 | 1.9 | - |
formaldehyde | - |
Paraburkholderia xenovorans LB400 | |
1.2.1.5 | 4.15 | - |
benzaldehyde | - |
Paraburkholderia xenovorans LB400 | |
1.2.1.77 | 0.04 | - |
NADP+ | in 50 mM Tris-HCl (pH 7.5), at 25°C | Paraburkholderia xenovorans | |
1.2.1.77 | 0.042 | - |
heptaldehyde | in 50 mM Tris-HCl (pH 7.5), at 25°C | Paraburkholderia xenovorans | |
1.2.1.77 | 0.3 | - |
Valeraldehyde | in 50 mM Tris-HCl (pH 7.5), at 25°C | Paraburkholderia xenovorans | |
1.2.1.77 | 0.501 | - |
NAD+ | in 50 mM Tris-HCl (pH 7.5), at 25°C | Paraburkholderia xenovorans | |
1.2.1.77 | 1.21 | - |
propionaldehyde | in 50 mM Tris-HCl (pH 7.5), at 25°C | Paraburkholderia xenovorans | |
1.2.1.77 | 1.66 | - |
Isovaleraldehyde | in 50 mM Tris-HCl (pH 7.5), at 25°C | Paraburkholderia xenovorans | |
1.2.1.77 | 1.9 | - |
formaldehyde | in 50 mM Tris-HCl (pH 7.5), at 25°C | Paraburkholderia xenovorans | |
1.2.1.77 | 4.15 | - |
benzaldehyde | in 50 mM Tris-HCl (pH 7.5), at 25°C | Paraburkholderia xenovorans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.5 | 110000 | - |
gel filtration | Paraburkholderia xenovorans LB400 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.77 | 3,4-dehydroadipyl-CoA semialdehyde + NADP+ + H2O | Paraburkholderia xenovorans | the enzyme is involved in the benzoate oxidation (box) pathway | 3,4-dehydroadipyl-CoA + NADPH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.5 | Paraburkholderia xenovorans | Q13WK4 | - |
- |
1.2.1.5 | Paraburkholderia xenovorans LB400 | Q13WK4 | - |
- |
1.2.1.77 | Paraburkholderia xenovorans | Q13WK4 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.5 | to homogeneity by nickel affinity chromatography and gel filtration | Paraburkholderia xenovorans LB400 |
1.2.1.77 | - |
Paraburkholderia xenovorans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.5 | benzaldehyde + NADP+ + H2O | - |
Paraburkholderia xenovorans LB400 | benzoate + NADPH + H+ | - |
? | |
1.2.1.5 | formaldehyde + NADP+ + H2O | - |
Paraburkholderia xenovorans LB400 | formate + NADPH + H+ | - |
? | |
1.2.1.5 | heptanal + NADP+ + H2O | - |
Paraburkholderia xenovorans LB400 | heptanoate + NADPH + H+ | - |
? | |
1.2.1.5 | isovaleraldehyde + NADP+ + H2O | - |
Paraburkholderia xenovorans LB400 | isovalerate + NADPH + H+ | - |
? | |
1.2.1.5 | additional information | ALDHC is preferentially active towards linear medium-chain to long-chain aldehydes as compared to branched-chain, short-chain or aromatic aldehydes. Reorientation of an extended loop (Asn478-Pro490) is responsible for the constricted structure of the substrate tunnel, with the side chain of Asn478 imposing steric restrictions on branched-chain and aromatic aldehydes. A key glycine (Gly104) positioned at the mouth of the tunnel allows for maximum tunnel depth required to bind the linear medium to long aliphatic chain of the native substrate | Paraburkholderia xenovorans LB400 | ? | - |
? | |
1.2.1.5 | propionaldehyde + NAD+ + H2O | - |
Paraburkholderia xenovorans LB400 | propionate + NADH + H+ | - |
r | |
1.2.1.5 | propionaldehyde + NAD+ + H2O | - |
Paraburkholderia xenovorans | propionate + NADH + H+ | - |
? | |
1.2.1.5 | propionaldehyde + NADP+ + H2O | - |
Paraburkholderia xenovorans LB400 | propionate + NADPH + H+ | - |
? | |
1.2.1.5 | valeraldehyde + NADP+ + H2O | - |
Paraburkholderia xenovorans LB400 | valerate + NADPH + H+ | - |
? | |
1.2.1.77 | 3,4-dehydroadipyl-CoA semialdehyde + NADP+ + H2O | the enzyme is involved in the benzoate oxidation (box) pathway | Paraburkholderia xenovorans | 3,4-dehydroadipyl-CoA + NADPH + H+ | - |
? | |
1.2.1.77 | 3,4-didehydroadipyl-CoA semialdehyde + NAD+ + H2O | - |
Paraburkholderia xenovorans | 3,4-didehydroadipyl-CoA + NADH + H+ | - |
r | |
1.2.1.77 | benzaldehyde + NADP+ + H2O | - |
Paraburkholderia xenovorans | benzoate + NADPH + H+ | - |
? | |
1.2.1.77 | formaldehyde + NADP+ + H2O | - |
Paraburkholderia xenovorans | formate + NADPH + H+ | - |
? | |
1.2.1.77 | heptaldehyde + NADP+ + H2O | - |
Paraburkholderia xenovorans | heptanoate + NADPH + H+ | - |
? | |
1.2.1.77 | isovaleraldehyde + NADP+ + H2O | - |
Paraburkholderia xenovorans | isovalerate + NADPH + H+ | - |
? | |
1.2.1.77 | additional information | the native substrate (3,4-dehydroadipyl-CoA semialdehyde) is not tested as it is commercially unavailable. The enzyme is preferentially active towards linear medium-chain to long-chain aldehydes as compared to branched-chain, short-chain or aromatic aldehydes | Paraburkholderia xenovorans | ? | - |
? | |
1.2.1.77 | propionaldehyde + NADP+ + H2O | - |
Paraburkholderia xenovorans | propionate + NADPH + H+ | - |
? | |
1.2.1.77 | valeraldehyde + NADP+ + H2O | - |
Paraburkholderia xenovorans | valerate + NADPH + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.1.5 | dimer | gel filtration | Paraburkholderia xenovorans LB400 |
1.2.1.77 | ? | forms a dimer in solution with a molecular mass of 110000, SDS-PAGE | Paraburkholderia xenovorans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.5 | aldehyde dehydrogenase | - |
Paraburkholderia xenovorans LB400 |
1.2.1.5 | ALDHC | - |
Paraburkholderia xenovorans LB400 |
1.2.1.5 | chromosomally encoded box pathway ALDH | - |
Paraburkholderia xenovorans LB400 |
1.2.1.77 | ALDHC | - |
Paraburkholderia xenovorans |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.77 | 0.019 | - |
benzaldehyde | in 50 mM Tris-HCl (pH 7.5), at 25°C | Paraburkholderia xenovorans | |
1.2.1.77 | 0.04 | - |
NADP+ | in 50 mM Tris-HCl (pH 7.5), at 25°C, cosubstrate: propionaldehyde | Paraburkholderia xenovorans | |
1.2.1.77 | 0.046 | - |
formaldehyde | in 50 mM Tris-HCl (pH 7.5), at 25°C | Paraburkholderia xenovorans | |
1.2.1.77 | 0.074 | - |
Isovaleraldehyde | in 50 mM Tris-HCl (pH 7.5), at 25°C | Paraburkholderia xenovorans | |
1.2.1.77 | 0.501 | - |
NAD+ | in 50 mM Tris-HCl (pH 7.5), at 25°C, cosubstrate: propionaldehyde | Paraburkholderia xenovorans | |
1.2.1.77 | 2.32 | - |
propionaldehyde | in 50 mM Tris-HCl (pH 7.5), at 25°C | Paraburkholderia xenovorans | |
1.2.1.77 | 5.16 | - |
Valeraldehyde | in 50 mM Tris-HCl (pH 7.5), at 25°C | Paraburkholderia xenovorans | |
1.2.1.77 | 29.9 | - |
heptaldehyde | in 50 mM Tris-HCl (pH 7.5), at 25°C | Paraburkholderia xenovorans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.5 | NAD+ | - |
Paraburkholderia xenovorans LB400 | |
1.2.1.5 | NADP+ | significant preference for NADP+. Cofactor selectivity is governed by a complex network of hydrogen bonds between the oxygen atoms of the 2'-phosphoryl moiety of NADP+ and a threonine/lysine pair on ALDHC | Paraburkholderia xenovorans LB400 | |
1.2.1.77 | NAD+ | the enzyme is more active in the presence of NADP+ relative to NAD+. Crystallographic data show that cofactor selectivity is governed by a complex network of hydrogen bonds between the oxygen atoms of the 2'-phosphoryl moiety of NADP+ and a threonine/lysine pair on the enzyme | Paraburkholderia xenovorans | |
1.2.1.77 | NADP+ | the enzyme is more active in the presence of NADP+ relative to NAD+. Crystallographic data show that cofactor selectivity is governed by a complex network of hydrogen bonds between the oxygen atoms of the 2'-phosphoryl moiety of NADP+ and a threonine/lysine pair on the enzyme | Paraburkholderia xenovorans |