EC Number | Cloned (Comment) | Organism |
---|---|---|
3.6.1.40 | expression of His-tagged Y19N mutant | Aquifex aeolicus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.6.1.40 | PPX/GPPA Y19N mutant enzyme in complex with guanosine tetraphosphate, hanging-drop vapor diffusion method by mixing 0.002 ml of protein and 0.002 ml of reservoir solution containing 200 mM sodium acetate, pH 6.4, 100 mM 4-morpholineethanesulfonic acid, pH 5.7, and 50% methyl-2,4-pentanediol, X-ray diffraction structure determination and analysis at 2.7 A resolution, modelling | Aquifex aeolicus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.6.1.40 | Y19N | site-directed mutagenesis, crystal structure | Aquifex aeolicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.40 | guanosine 5'-triphosphate,3'-diphosphate + H2O | Aquifex aeolicus | - |
guanosine 5'-diphosphate,3'-diphosphate + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.40 | Aquifex aeolicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.6.1.40 | recombinant His-tagged Y19N mutant by nickel affinity chromatography removal of His-tag, and gel filtration | Aquifex aeolicus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.6.1.40 | guanosine 5'-triphosphate 3'-diphosphate + H2O = guanosine 3',5'-bis(diphosphate) + phosphate | the hydrolytic base is E119, the dual specificity typical for the exopolyphosphatase/guanosine pentaphosphate phosphohydrolase protein family is due to a common active site for guanosine pentaphosphate and polyphosphate hydrolysis, R22 and R267, residing in different domains, are crucial for guanosine pentaphosphate specificity as they interact with the unique 3'-ribose phosphorylation | Aquifex aeolicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.40 | guanosine 5'-triphosphate,3'-diphosphate + H2O | - |
Aquifex aeolicus | guanosine 5'-diphosphate,3'-diphosphate + phosphate | - |
? | |
3.6.1.40 | guanosine 5'-triphosphate,3'-diphosphate + H2O | substrate binding structure and involved residues, overview | Aquifex aeolicus | guanosine 5'-diphosphate,3'-diphosphate + phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.1.40 | exopolyphosphatase/guanosine pentaphosphate phosphohydrolase | - |
Aquifex aeolicus |
3.6.1.40 | More | the enzyme belongs to the exopolyphosphatase/guanosine pentaphosphate phosphohydrolase protein family | Aquifex aeolicus |
3.6.1.40 | PPX/GPPA enzyme | - |
Aquifex aeolicus |