Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tanaka, S.; Matsumura, H.; Koga, Y.; Takano, K.; Kanaya, S.
    Four new crystal structures of Tk-subtilisin in unautoprocessed, autoprocessed and mature forms: insight into structural changes during maturation (2007), J. Mol. Biol., 372, 1055-1069.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.21.62 by sitting-drop, vapor-diffusion method. Crystal structures of the autoprocessed/unautoprocessed form and mature forms of subtilisin at 1.89 A and 1.70 A resolution, respectively. Crystals show a unique Ca2+-binding site and that the N-terminal region of the mature domain (Gly70–Pro82), which binds tightly to the main body in the unautoprocessed form, is disordered and mostly truncated in the autoprocessed and mature forms, respectively. Crystal structure of the propeptide:S324A-subtilisin complex at 1.65 a resolution Thermococcus kodakarensis

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.62 S324A pro-subtilisin, which is not autoprocessed at all when the active site serine residue is replaced with Ala. Is refolded in the presence of Ca2+ Thermococcus kodakarensis
3.4.21.62 S324C propeptide:subtilisin complex is stable Thermococcus kodakarensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.62 diisopropylphosphofluoridate
-
 Thermococcus kodakarensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.62 Ca2+ calcium ion binds weakly to the Ca-7 site in the unautoprocessed form, but is trapped upon autoprocessing. The Ca-7 site is required to promote the autoprocessing reaction by stabilizing the autoprocessed form, in which the new N-terminus of the mature domain is structurally disordered Thermococcus kodakarensis

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.62 Thermococcus kodakarensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.62 S324A-subtilisin and propeptide, by gel filtration Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.62 N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
-
 Thermococcus kodakarensis N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.62 80
-
-
 Thermococcus kodakarensis