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Literature summary extracted from
- Structure of zebra fish HIUase: insights into evolution of an enzyme to a hormone transporter (2006), J. Mol. Biol., 363, 1-9.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.2.17 | expressed in Escherichia coli BL21 (DE3) cells | Danio rerio |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.5.2.17 | at 4°C by the sitting-drop, vapour diffusion method, using 0.1 M HEPES (pH 7.5), 20% (w/v) PEG 10000 | Danio rerio |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.5.2.17 | D50N | shows 50% activity of the wild type enzyme | Danio rerio |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.2.17 | Danio rerio | Q06S87 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.2.17 | Q-Sepharose column chromatography | Danio rerio |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.2.17 | 5-hydroxyisourate + H2O | - |
Danio rerio | 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate | - |
? |  |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.2.17 | homotetramer | x-ray crystallography | Danio rerio |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.2.17 | 5-hydroxyisourate hydrolase | - |
Danio rerio |
| 3.5.2.17 | HIUase | - |
Danio rerio |
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Release 2023.1 (January 2023)
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