EC Number | Cloned (Comment) | Organism |
---|---|---|
1.15.1.1 | DNA and amino acid sequence determnination and analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Rhodothermus sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.15.1.1 | H2O2 | inhibition at 10 mM, the Fe-SOD is less sensitive | Rhodothermus sp. | |
1.15.1.1 | KCN | inhibition at 10 mM, the Fe-SOD is less sensitive | Rhodothermus sp. | |
1.15.1.1 | NaN3 | inhibition at 5 mM, the Fe-SOD is less sensitive | Rhodothermus sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.15.1.1 | Fe2+ | a Fe-SOD | Rhodothermus sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.15.1.1 | 23600 | - |
2 * 23600, about, recombinant enzyme, sequence calcualtion, 2 * 25000, recombinant enzyme, SDS-PAGE | Rhodothermus sp. |
1.15.1.1 | 25000 | - |
2 * 23600, about, recombinant enzyme, sequence calcualtion, 2 * 25000, recombinant enzyme, SDS-PAGE | Rhodothermus sp. |
1.15.1.1 | 52000 | - |
recombinant enzyme, gel filtration | Rhodothermus sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.15.1.1 | O2.- + H+ | Rhodothermus sp. | - |
O2 + H2O2 | - |
? | |
1.15.1.1 | O2.- + H+ | Rhodothermus sp. XMH10 | - |
O2 + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.15.1.1 | Rhodothermus sp. | - |
- |
- |
1.15.1.1 | Rhodothermus sp. XMH10 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.15.1.1 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Rhodothermus sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.15.1.1 | O2.- + H+ | - |
Rhodothermus sp. | O2 + H2O2 | - |
? | |
1.15.1.1 | O2.- + H+ | - |
Rhodothermus sp. XMH10 | O2 + H2O2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.15.1.1 | dimer | 2 * 23600, about, recombinant enzyme, sequence calcualtion, 2 * 25000, recombinant enzyme, SDS-PAGE | Rhodothermus sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.15.1.1 | SOD | - |
Rhodothermus sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.15.1.1 | 50 | 70 | recombinant enzyme | Rhodothermus sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.15.1.1 | 70 | - |
12 h, purified recombinant enzyme, 90% remaining activity | Rhodothermus sp. |
1.15.1.1 | 80 | - |
purified recombinant enzyme, 40% remaining activity after 30 min, complete inactivation after 4 h | Rhodothermus sp. |
1.15.1.1 | 90 | - |
purified recombinant enzyme, 10% remaining activity after 30 min | Rhodothermus sp. |
1.15.1.1 | 100 | - |
purified recombinant enzyme, complete inactivation after 30 min | Rhodothermus sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.15.1.1 | 5 | - |
recombinant enzyme | Rhodothermus sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.15.1.1 | 4 | 12 | inactivation at pH 2.0, shrap drop of activity below pH 4.0, high activity at pH 4.0-pH 5.0, about 70% of maximal activity at pH 6.0-12.0 | Rhodothermus sp. |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.15.1.1 | Rhodothermus sp. | sequence calcualtion | - |
5.54 |