EC Number | Cloned (Comment) | Organism |
---|---|---|
1.4.3.3 | expressed in Escherichia coli BL21(DE3)pLysS cells | Rhodotorula toruloides |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.4.3.3 | M213E | increased overall activity compared to the wild type enzyme | Rhodotorula toruloides |
1.4.3.3 | M213G | increased overall activity compared to the wild type enzyme | Rhodotorula toruloides |
1.4.3.3 | M213R | increased overall activity compared to the wild type enzyme | Rhodotorula toruloides |
1.4.3.3 | Q144R | increased overall activity compared to the wild type enzyme | Rhodotorula toruloides |
1.4.3.3 | Q339E | decreased overall activity compared to the wild type enzyme | Rhodotorula toruloides |
1.4.3.3 | T60A/Q144R/K152E | increased overall activity compared to the wild type enzyme | Rhodotorula toruloides |
1.4.3.3 | Y238R | increased overall activity compared to the wild type enzyme | Rhodotorula toruloides |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.3 | 0.3 | - |
D-methionine | mutant enzyme T60A/Q144R/K152E, apparent value, at 25°C and pH 8.5 | Rhodotorula toruloides | |
1.4.3.3 | 0.6 | - |
D-alanine | mutant enzyme T60A/Q144R/K152E, apparent value, at 25°C and pH 8.5 | Rhodotorula toruloides | |
1.4.3.3 | 0.6 | - |
D-methionine | wild type enzyme, apparent value, at 25°C and pH 8.5 | Rhodotorula toruloides | |
1.4.3.3 | 0.8 | - |
D-methionine | mutant enzyme M213G, apparent value, at 25°C and pH 8.5 | Rhodotorula toruloides | |
1.4.3.3 | 0.9 | - |
D-alanine | wild type enzyme, apparent value, at 25°C and pH 8.5 | Rhodotorula toruloides | |
1.4.3.3 | 1.2 | - |
D-methionine | mutant enzyme M213R, apparent value, at 25°C and pH 8.5 | Rhodotorula toruloides | |
1.4.3.3 | 1.3 | - |
D-valine | immobilized wild type enzyme, at 25°C | Rhodotorula toruloides | |
1.4.3.3 | 2.5 | - |
D-glutamine | mutant enzyme M213G, apparent value, at 25°C and pH 8.5 | Rhodotorula toruloides | |
1.4.3.3 | 4.9 | - |
D-alanine | mutant enzyme M213G, apparent value, at 25°C and pH 8.5 | Rhodotorula toruloides | |
1.4.3.3 | 5.1 | - |
D-glutamate | mutant enzyme M213G, apparent value, at 25°C and pH 8.5 | Rhodotorula toruloides | |
1.4.3.3 | 5.4 | - |
D-glutamine | mutant enzyme M213R, apparent value, at 25°C and pH 8.5 | Rhodotorula toruloides | |
1.4.3.3 | 5.4 | - |
D-glutamine | wild type enzyme, apparent value, at 25°C and pH 8.5 | Rhodotorula toruloides | |
1.4.3.3 | 5.6 | - |
D-proline | immobilized wild type enzyme, at 25°C | Rhodotorula toruloides | |
1.4.3.3 | 6 | - |
D-glutamine | mutant enzyme T60A/Q144R/K152E, apparent value, at 25°C and pH 8.5 | Rhodotorula toruloides | |
1.4.3.3 | 8 | - |
D-Lysine | mutant enzyme M213G, apparent value, at 25°C and pH 8.5 | Rhodotorula toruloides | |
1.4.3.3 | 12.5 | - |
D-proline | free wild type enzyme, at 25°C | Rhodotorula toruloides | |
1.4.3.3 | 14.6 | - |
D-Lysine | wild type enzyme, apparent value, at 25°C and pH 8.5 | Rhodotorula toruloides | |
1.4.3.3 | 16.6 | - |
D-Lysine | mutant enzyme T60A/Q144R/K152E, apparent value, at 25°C and pH 8.5 | Rhodotorula toruloides | |
1.4.3.3 | 18.5 | - |
D-alanine | mutant enzyme M213R, apparent value, at 25°C and pH 8.5 | Rhodotorula toruloides | |
1.4.3.3 | 18.9 | - |
D-valine | free wild type enzyme, at 25°C | Rhodotorula toruloides | |
1.4.3.3 | 21.1 | - |
D-glutamate | mutant enzyme M213R, apparent value, at 25°C and pH 8.5 | Rhodotorula toruloides | |
1.4.3.3 | 76 | - |
D-glutamate | wild type enzyme, apparent value, at 25°C and pH 8.5 | Rhodotorula toruloides | |
1.4.3.3 | 101 | - |
D-glutamate | mutant enzyme T60A/Q144R/K152E, apparent value, at 25°C and pH 8.5 | Rhodotorula toruloides |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.3.3 | Rhodotorula toruloides | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.3.3 | Hitrap chelating chromatography | Rhodotorula toruloides |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.3.3 | cephalosporin C + H2O + O2 | - |
Rhodotorula toruloides | 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-alanine + H2O + O2 | - |
Rhodotorula toruloides | pyruvate + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-arginine + H2O + O2 | - |
Rhodotorula toruloides | 5-guanidino-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-asparagine + H2O + O2 | - |
Rhodotorula toruloides | 2-oxosuccinamic acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-aspartate + H2O + O2 | - |
Rhodotorula toruloides | oxaloacetate + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-cysteine + H2O + O2 | - |
Rhodotorula toruloides | 2-oxo-3-thiopropionic acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-glutamate + H2O + O2 | - |
Rhodotorula toruloides | alpha-ketoglutarate + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-glutamine + H2O + O2 | - |
Rhodotorula toruloides | 2-oxoglutaramate + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-histidine + H2O + O2 | - |
Rhodotorula toruloides | 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-isoleucine + H2O + O2 | - |
Rhodotorula toruloides | 3-methyl-2-oxopentanoate + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-leucine + H2O + O2 | - |
Rhodotorula toruloides | 4-methyl-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-lysine + H2O + O2 | - |
Rhodotorula toruloides | 6-amino-2-oxohexanoic acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-methionine + H2O + O2 | - |
Rhodotorula toruloides | 4-methylthio-2-oxobutanoic acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-phenylalanine + H2O + O2 | - |
Rhodotorula toruloides | phenylpyruvate + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-proline + H2O + O2 | - |
Rhodotorula toruloides | 2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-serine + H2O + O2 | - |
Rhodotorula toruloides | 2-oxo-3-hydroxypropionic acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-threonine + H2O + O2 | - |
Rhodotorula toruloides | 2-oxo-3-hydroxybutyric acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-tryptophan + H2O + O2 | - |
Rhodotorula toruloides | indol-3-pyruvate + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-tyrosine + H2O + O2 | - |
Rhodotorula toruloides | 4-hydroxyphenylpyruvic acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-valine + H2O + O2 | - |
Rhodotorula toruloides | alpha-ketoisovaleric acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | glycine + 2 H2O + O2 | - |
Rhodotorula toruloides | 2 formic acid + NH3 + H2O2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.3.3 | D-amino acid oxidase | - |
Rhodotorula toruloides |
1.4.3.3 | DAAO | - |
Rhodotorula toruloides |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.3.3 | FAD | FAD-containing flavoenzyme | Rhodotorula toruloides |