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Literature summary extracted from

  • Yalin, Y.; Jin, L.; Jianhua, W.; Da, T.; Zigang, T.
    Expression and characterization of dextransucrase gene dsrX from Leuconostoc mesenteroides in Escherichia coli (2008), J. Biotechnol., 133, 505-512.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.5 gene dsrX, DNA and amino acid sequence determination and analysis, cloning in Escherichia coli strain DH5alpha, expressed as a N-terminal His6-tagged protein Escherichia coli strain BL21(DE3), optimization of the recombinant expression system Leuconostoc mesenteroides

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.5 Al3+ strongly inhibits the native and the recombinant enzyme Leuconostoc mesenteroides
2.4.1.5 Cu2+ strongly inhibits the native and the recombinant enzyme Leuconostoc mesenteroides
2.4.1.5 EDTA inhibits the native and the recombinant enzyme Leuconostoc mesenteroides
2.4.1.5 Fe2+ slightly inhibits the native, but not the recombinant enzyme Leuconostoc mesenteroides
2.4.1.5 Mg2+ slightly inhibits the native, but not the recombinant enzyme Leuconostoc mesenteroides
2.4.1.5 Na+ slightly inhibits the native, but not the recombinant enzyme Leuconostoc mesenteroides
2.4.1.5 Ni2+ slightly inhibits the native and the recombinant enzyme Leuconostoc mesenteroides
2.4.1.5 SDS inhibits the native and the recombinant enzyme Leuconostoc mesenteroides
2.4.1.5 Zn2+ slightly inhibits the native and the recombinant enzyme Leuconostoc mesenteroides

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.5 Co2+ slightly activates the native and the recombinant enzyme Leuconostoc mesenteroides
2.4.1.5 Mn2+ activates the native and the recombinant enzyme Leuconostoc mesenteroides
2.4.1.5 additional information the enzyme is not or poorly affected by Ca2+, K+, and pepstatin Leuconostoc mesenteroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.5 167570
-
 x * 167570, recombinant His6-tagged DsrX, sequence calculation Leuconostoc mesenteroides

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.5 Leuconostoc mesenteroides
-
 gene dsrX
-
2.4.1.5 Leuconostoc mesenteroides CGMCC 1.544
-
 gene dsrX
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.5 native enzyme partially by ammonium sulfate fractionation and dialysis, recombinant N-terminally His6-tagged DsrX from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Leuconostoc mesenteroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.5 97.37
-
 recombinant enzyme in crude enzyme extract Leuconostoc mesenteroides

Subunits

EC Number Subunits Comment Organism
2.4.1.5 ? x * 167570, recombinant His6-tagged DsrX, sequence calculation Leuconostoc mesenteroides

Synonyms

EC Number Synonyms Comment Organism
2.4.1.5 DsrX
-
 Leuconostoc mesenteroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.5 30
-
 recombinant enzyme Leuconostoc mesenteroides
2.4.1.5 40
-
 native enzyme Leuconostoc mesenteroides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.5 4 60 the native DsrX shows higher stability than the recombinant DsrX at the temperature range from 4°C to 60°C for 2 h, native enzyme shows 84% remaining activity at 40°C after 2 h, while recombinant enzyme shows only 3.1% Leuconostoc mesenteroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.5 5.4
-
 native and recombinant enzyme Leuconostoc mesenteroides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.5 3 8
-
 Leuconostoc mesenteroides

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.5 4 5 purified recombinant enzyme, quite stable Leuconostoc mesenteroides