Literature summary extracted from

  • Zhu, D.; Mukherjee, C.; Yang, Y.; Rios, B.E.; Gallagher, D.T.; Smith, N.N.; Biehl, E.R.; Hua, L.
    A new nitrilase from Bradyrhizobium japonicum USDA 110. Gene cloning, biochemical characterization and substrate specificity (2008), J. Biotechnol., 133, 327-333.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.5.5 expressed in Escherichia coli Rosetta2 (DE3) cells Bradyrhizobium japonicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.5.5 4.36
-
phenylacetonitrile
-
Bradyrhizobium japonicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.5.5 340000
-
holoenzyme, light scattering analysis Bradyrhizobium japonicum
3.5.5.5 34000
-
10 * 34000, holoenzyme, light scattering analysis Bradyrhizobium japonicum
3.5.5.5 34500
-
10 * 34500, subunit, SDS-PAGE Bradyrhizobium japonicum

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.5 Bradyrhizobium japonicum
-
-
-
3.5.5.5 Bradyrhizobium japonicum USDA 110
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.5 DEAE Sepharose Fast Flow column chromatography and Sephacryl S-300 gel filtration Bradyrhizobium japonicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.5 additional information does not use 2-(N,N-dimethylamino)-2-phenylacetonitrile, 2-phenylpropionitrile, and 2-phenylbutyronitrile as substrate Bradyrhizobium japonicum ?
-
?
3.5.5.5 additional information does not use 2-(N,N-dimethylamino)-2-phenylacetonitrile, 2-phenylpropionitrile, and 2-phenylbutyronitrile as substrate Bradyrhizobium japonicum USDA 110 ?
-
?
3.5.5.5 mandelonitrile + 2 H2O 4% activity compared to phenylacetonitrile Bradyrhizobium japonicum mandelic acid + NH3
-
?
3.5.5.5 mandelonitrile + 2 H2O 4% activity compared to phenylacetonitrile Bradyrhizobium japonicum USDA 110 mandelic acid + NH3
-
?
3.5.5.5 4-chlorobutyronitrile + H2O 59% activity compared to phenylacetonitrile Bradyrhizobium japonicum 4-chlorobutyrate + NH3
-
?
3.5.5.5 4-chlorobutyronitrile + H2O 59% activity compared to phenylacetonitrile Bradyrhizobium japonicum USDA 110 4-chlorobutyrate + NH3
-
?
3.5.5.5 n-butyronitrile + H2O 15% activity compared to phenylacetonitrile Bradyrhizobium japonicum n-butyric acid + NH3
-
?
3.5.5.5 n-butyronitrile + H2O 15% activity compared to phenylacetonitrile Bradyrhizobium japonicum USDA 110 n-butyric acid + NH3
-
?
3.5.5.5 phenylacetonitrile + 2 H2O 100% activity Bradyrhizobium japonicum phenylacetic acid + NH3
-
?
3.5.5.5 phenylacetonitrile + 2 H2O 100% activity Bradyrhizobium japonicum USDA 110 phenylacetic acid + NH3
-
?
3.5.5.5 3-hydroxypropionitrile + H2O 1% activity compared to phenylacetonitrile Bradyrhizobium japonicum 3-hydroxypropanoate + NH3
-
?
3.5.5.5 crotononitrile + 2 H2O 4% activity compared to phenylacetonitrile Bradyrhizobium japonicum crotonic acid + NH3
-
?
3.5.5.5 valeronitrile + H2O 57% activity compared to phenylacetonitrile Bradyrhizobium japonicum valeric acid + NH3
-
?
3.5.5.5 1,4-dicyanobutane + H2O 47% activity compared to phenylacetonitrile Bradyrhizobium japonicum adipic acid + NH3
-
?
3.5.5.5 2-methylglutaronitrile + H2O 63% activity compared to phenylacetonitrile Bradyrhizobium japonicum ? + NH3
-
?
3.5.5.5 2-phenylglycinonitrile + 2 H2O 3% activity compared to phenylacetonitrile Bradyrhizobium japonicum 2-phenylglycine + NH3
-
?
3.5.5.5 2-trimethylsilyloxy-2-phenylacetonitrile + H2O 4% activity compared to phenylacetonitrile Bradyrhizobium japonicum 2-trimethylsilyloxy-2-phenylacetic acid + NH3
-
?
3.5.5.5 3-aminopropionitrile + H2O 7% activity compared to phenylacetonitrile Bradyrhizobium japonicum 3-aminopropanoic acid + NH3
-
?
3.5.5.5 3-indolylacetonitrile + H2O 2% activity compared to phenylacetonitrile Bradyrhizobium japonicum 3-indolylacetic acid + NH3
-
?
3.5.5.5 4-phenylbutyronitrile + H2O 86% activity compared to phenylacetonitrile Bradyrhizobium japonicum 4-phenylbutyrate + NH3
-
?
3.5.5.5 allyl cyanide + NH3 11% activity compared to phenylacetonitrile Bradyrhizobium japonicum 3-butenoic acid + NH3
-
?
3.5.5.5 benzonitrile + H2O 1% activity compared to phenylacetonitrile Bradyrhizobium japonicum benzoic acid + NH3
-
?
3.5.5.5 heptanenitrile + H2O 107% activity compared to phenylacetonitrile Bradyrhizobium japonicum heptanoic acid + NH3
-
?
3.5.5.5 hexanenitrile + H2O 60% activity compared to phenylacetonitrile Bradyrhizobium japonicum hexanoic acid + NH3
-
?
3.5.5.5 4-hydroxycinnamonitrile + H2O 431% activity compared to phenylacetonitrile Bradyrhizobium japonicum 4-hydroxycinnamic acid + NH3
-
?
3.5.5.5 methylthioacetonitrile + H2O 72% activity compared to phenylacetonitrile Bradyrhizobium japonicum methylthioacetic acid + NH3
-
?

Subunits

EC Number Subunits Comment Organism
3.5.5.5 homodecamer 10 * 34000, holoenzyme, light scattering analysis Bradyrhizobium japonicum
3.5.5.5 homodecamer 10 * 34500, subunit, SDS-PAGE Bradyrhizobium japonicum

Synonyms

EC Number Synonyms Comment Organism
3.5.5.5 blr3397
-
Bradyrhizobium japonicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.5.5 45
-
-
Bradyrhizobium japonicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.5.5 50 70 good thermal stability at 50°C, the enzyme completely loses its activity after heat-treatment at 70°C for 30 min Bradyrhizobium japonicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.5.5 1.85
-
phenylacetonitrile
-
Bradyrhizobium japonicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.5.5 7 8
-
Bradyrhizobium japonicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.5.5 5 9
-
Bradyrhizobium japonicum