Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rodrigues, J.V.; Victor, B.L.; Huber, H.; Saraiva, L.M.; Soares, C.M.; Cabelli, D.E.; Teixeira, M.
    Superoxide reduction by Nanoarchaeum equitans neelaredoxin, an enzyme lacking the highly conserved glutamate iron ligand (2008), J. Biol. Inorg. Chem., 13, 219-228.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.2 expression of wild-type and mutant enzymes in Escherichia coli strain XL2Blue Nanoarchaeum equitans

Protein Variants

EC Number Protein Variants Comment Organism
1.15.1.2 P8E site-directed mutagenesis, pH-induced transition of the mutant is similar to the wild-type enzyme, the reactivity of the N. equitans P8E Nlr mutant towards superoxide measured at different pHs is identical to that of the wild-type protein Nanoarchaeum equitans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.15.1.2 additional information
-
 additional information the reaction with superoxide proceeds via formation of an intermediate with a second-order rate constant Nanoarchaeum equitans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.2 Fe2+ 1Fe-SOR or neelaredoxin class of superoxide reductases Nanoarchaeum equitans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.15.1.2 reduced rubredoxin + superoxide + H+ Nanoarchaeum equitans superoxide scavenging by superoxide reductases constitutes an alternative detoxifying system to the canonical superoxide dismutases, instead of dismutating superoxide, SORs catalyse only the reductive reaction, forming hydrogen peroxide as a product rubredoxin + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.2 Nanoarchaeum equitans
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.15.1.2 superoxide + reduced rubredoxin + 2 H+ = H2O2 + oxidized rubredoxin reaction mechanism involves the diffusion-limited encounter of superoxide with the reduced iron site and concomitant formation of an Fe3+-(hydro)peroxo adduct that, upon protonation, leads to the formation of hydrogen peroxide. By the end of this process, a glutamate residue coordinates the ferric ion, acting as a sixth ligand Nanoarchaeum equitans
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.2 reduced rubredoxin + superoxide + H+
-
 Nanoarchaeum equitans rubredoxin + H2O2
-
 ?
1.15.1.2 reduced rubredoxin + superoxide + H+ superoxide scavenging by superoxide reductases constitutes an alternative detoxifying system to the canonical superoxide dismutases, instead of dismutating superoxide, SORs catalyse only the reductive reaction, forming hydrogen peroxide as a product Nanoarchaeum equitans rubredoxin + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.15.1.2 More three-dimensional modelling of the enzyme Nanoarchaeum equitans

Synonyms

EC Number Synonyms Comment Organism
1.15.1.2 1Fe-SOR
-
 Nanoarchaeum equitans
1.15.1.2 neelaredoxin
-
 Nanoarchaeum equitans
1.15.1.2 Nlr
-
 Nanoarchaeum equitans
1.15.1.2 SOR
-
 Nanoarchaeum equitans

Cofactor

EC Number Cofactor Comment Organism Structure
1.15.1.2 reduced rubredoxin
-
 Nanoarchaeum equitans