EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.169 | expression in Escherichia coli | Moorella thermoacetica |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.7.4 | 72000 | - |
beta subunit, SDS-PAGE | Methanosarcina thermophila |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.7.4 | Methanosarcina thermophila | - |
strain DSM 521 | - |
1.2.7.4 | Moorella thermoacetica | - |
strain DSM 521 | - |
2.3.1.169 | Moorella thermoacetica | P27988 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.7.4 | - |
Moorella thermoacetica |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.3.1.169 | acetyl-CoA + a [Co(I) corrinoid Fe-S protein] = CO + CoA + a [methyl-Co(III) corrinoid Fe-S protein] | CoA is the last substrate to bind and CO and the methyl group bind randomly as the first substrate in acetyl-CoA synthesis | Moorella thermoacetica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.7.4 | CO + H2O + acceptor | - |
Moorella thermoacetica | CO2 + reduced acceptor | - |
r | |
1.2.7.4 | CO + H2O + acceptor | - |
Methanosarcina thermophila | CO2 + reduced acceptor | - |
? | |
2.3.1.169 | additional information | CoA is the last substrate to bind and CO and the methyl group bind randomly as the first substrate in acetyl-CoA synthesis. In pulse-chase experiments, up to 100% of the methyl groups and CoA and up to 60-70% of the CO employed in the pulse phase can be trapped in the product acetyl-CoA | Moorella thermoacetica | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.7.4 | CODH | beta subunit of the bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase complex | Methanosarcina thermophila |
1.2.7.4 | CODHACS | bifunctional enzyme, CODHACS is a macromolecular machine that catalyzes the last step of the Wood-Ljungdahl pathway of anaerobic carbon dioxide fixation | Moorella thermoacetica |