Literature summary extracted from

Bissonnette, S.A.; Glazier, C.M.; Stewart, M.Q.; Brown, G.E.; Ellson, C.D.; Yaffe, M.B.
Phosphatidylinositol 3-phosphate-dependent and -independent functions of p40phox in activation of the neutrophil NADPH oxidase (2008), J. Biol. Chem., 283, 2108-2119.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
1.6.3.1	phosphatidylinositol 3-phosphate	subunit p40phox phosphatidylinositol 3-phosphate binding PX domain has phosphatidylinositol 3-phosphate-dependent and -independent functions. Translocation of subunit p67phox requires the PX domain but not 3-phosphoinositide binding. Activation of the oxidase by p40phox, however, requires both phosphatidylinositol 3-phosphate binding and an Src homology 3 domain competent to bind to poly-Pro ligands	Homo sapiens

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.6.3.1	additional information	depletion of endogenous subunit p40phoxusing lentiviral short hairpin RNA reduces reactive oxygen species production and impairs bacterial killing by phagolysosomes under conditions where subunit p67phox levels remain constant. Depletion of p40phox reduces both the maximal rate and total amount of ROS produced without altering theKM value of the oxidase forNADPH	Homo sapiens

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.6.3.1	0.015	-	NADPH	subunit p40phox-depleted cytosol in the absencee of recombinant wild-type p40phox, pH 7.3, 37°C	Homo sapiens
1.6.3.1	0.024	-	NADPH	subunit p40phox-depleted cytosol in the presence of recombinant wild-type p40phox, pH 7.3, 37°C	Homo sapiens

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.6.3.1	Homo sapiens	-	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.6.3.1	PLB-985 cell	-	Homo sapiens	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.6.3.1	NADPH + H+ + O2	-	Homo sapiens	NADP+ + H2O2	-	?

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Release 2023.1 (January 2023)