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Literature summary extracted from

  • Belyaeva, O.V.; Johnson, M.P.; Kedishvili, N.Y.
    Kinetic analysis of human enzyme RDH10 defines the characteristics of a physiologically relevant retinol dehydrogenase (2008), J. Biol. Chem., 283, 20299-20308.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.315 expression in Sf9 cell Homo sapiens
1.1.1.315 RDH10 is expressed the enzyme in insect Sf9 cells using the Baculovirus expression system. Purification of RDH10-His6 from Sf9 cells using nickel affinity chromatography produces an inactive enzyme. Therefore, microsomal preparations of RDH10 are used for its kinetic characterization Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.315 11-cis-retinol substrate inhibition above 0.005 mM Homo sapiens
1.1.1.315 9-cis-retinol substrate inhibition above 0.001 mM Homo sapiens
1.1.1.315 all-trans-retinol substrate inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.315 0.035
-
 all-trans-retinol
-
 Homo sapiens
1.1.1.315 0.035
-
 all-trans-retinol microsomal preparations of RDH10, cofactor: NAD+ Homo sapiens
1.1.1.315 0.04
-
 9-cis-retinol microsomal preparations of RDH10 Homo sapiens
1.1.1.315 0.06
-
 11-cis-retinol microsomal preparations of RDH10 Homo sapiens
1.1.1.315 0.1
-
 NAD+
-
 Homo sapiens
1.1.1.315 0.1
-
 NAD+ microsomal preparations of RDH10 Homo sapiens
1.1.1.315 0.4
-
 all-trans-retinal
-
 Homo sapiens
1.1.1.315 0.4
-
 all-trans-retinal microsomal preparations of RDH10 Homo sapiens
1.1.1.315 6
-
 9-cis-retinol
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.315 39600
-
 x * 39600, SDS-PAGE and calculated Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.315 additional information Homo sapiens dual physiological role of isoform RDH10: in the biosynthesis of 11-cis-retinaldehyde for vision and in the biosynthesis of all-trans-retinoic acid for differentiation and development ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.315 Homo sapiens
-
 isoform Rdh10
-
1.1.1.315 Homo sapiens Q8IZV5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.315 purification of His-tagged enzyme using nickel affinity chromatography results in an inactive enzyme Homo sapiens

Storage Stability

EC Number Storage Stability Organism
1.1.1.315 -20°C, some loss of activity after repeated freeze-thawing cycles Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.315 11-cis-retinol + NAD+ microsomal preparations of RDH10 are not active in presence of NADP+ Homo sapiens 11-cis-retinal + NADH + H+
-
 r
1.1.1.315 11-cis-retinol + NADP+
-
 Homo sapiens 11-cis-retinal + NADPH
-
 ?
1.1.1.315 9-cis-retinol + NAD+ microsomal preparations of RDH10 are not active in presence of NADP+ Homo sapiens 9-cis-retinal + NADH + H+
-
 r
1.1.1.315 9-cis-retinol + NADP+
-
 Homo sapiens 9-cis-retinal + NADPH
-
 ?
1.1.1.315 all-trans-retinal + NADPH + H+
-
 Homo sapiens all-tans-retinol + NADP+
-
 r
1.1.1.315 all-trans-retinol + NAD+ RDH10 is a more efficient retinol dehydrogenase than a retinaldehyde reductase. Microsomal preparations of RDH10 are not active in presence of NADP+ Homo sapiens all-trans-retinal + NADH + H+
-
 r
1.1.1.315 all-trans-retinol + NADPH
-
 Homo sapiens all-trans-retinal + NADP+
-
 r
1.1.1.315 additional information dual physiological role of isoform RDH10: in the biosynthesis of 11-cis-retinaldehyde for vision and in the biosynthesis of all-trans-retinoic acid for differentiation and development Homo sapiens ?
-
 ?
1.1.1.315 additional information enzyme does not recognizes retinol bound to cellular retinol-binding protein type I as a substrate and functions exclusively in the oxidative reaction in cells Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.315 ? x * 39600, SDS-PAGE and calculated Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.1.1.315 RDH10
-
 Homo sapiens
1.1.1.315 retinol dehydrogenase 10
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.315 NAD+ isoform RDH10 is strictly NAD+-dependent Homo sapiens
1.1.1.315 NAD+ microsomal preparations of RDH10 are not active in presence of NADP+ Homo sapiens
1.1.1.315 NADH
-
 Homo sapiens