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Literature summary extracted from

  • Ding, Y.; Williams, R.M.; Sherman, D.H.
    Molecular analysis of a 4-dimethylallyltryptophan synthase from Malbranchea aurantiaca (2008), J. Biol. Chem., 283, 16068-16076.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.34 expression in Escherichia coli Malbranchea aurantiaca

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.34 Co2+ 5 mM, 50-80% inhibition Malbranchea aurantiaca
2.5.1.34 D-tryptophan competitive with L-tryptophan Malbranchea aurantiaca
2.5.1.34 EDTA 5 mM, inactivation. Regeneration of activity by dialysis with reaction buffer ontaining MgCl2 Malbranchea aurantiaca
2.5.1.34 Fe2+ 5 mM, 50-80% inhibition Malbranchea aurantiaca
2.5.1.34 Mn2+ 5 mM, 50-80% inhibition Malbranchea aurantiaca
2.5.1.34 Ni2+ 5 mM, 50-80% inhibition Malbranchea aurantiaca
2.5.1.34 Zn2+ 5 mM, 50-80% inhibition Malbranchea aurantiaca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.34 0.04888
-
 L-tryptophan pH 7.4, 30°C Malbranchea aurantiaca
2.5.1.34 0.106
-
 Nalpha-methyl-L-tryptophan pH 7.4, 30°C Malbranchea aurantiaca
2.5.1.34 0.1078
-
 L-tryptophan presence of D-tryptophan, pH 7.4, 30°C Malbranchea aurantiaca
2.5.1.34 0.3031
-
 5-hydroxy-L-tryptophan pH 7.4, 30°C Malbranchea aurantiaca

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.34 Ca2+ stimulation to 130% of initial activity Malbranchea aurantiaca
2.5.1.34 Mg2+ stimulation to 130% of initial activity Malbranchea aurantiaca
2.5.1.34 additional information activity does not depend on addition of divalent cation cofactor Malbranchea aurantiaca

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.34 51000
-
 1 * 52000, calculated, 1 * 51000, SDS-PAGE, His-tagged protein Malbranchea aurantiaca
2.5.1.34 52000
-
 1 * 52000, calculated, 1 * 51000, SDS-PAGE, His-tagged protein Malbranchea aurantiaca
2.5.1.34 61600
-
 gel filtration Malbranchea aurantiaca

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.34 Malbranchea aurantiaca B1A4C4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.34 dimethylallyl diphosphate + 1-methyl-L-tryptophan
-
 Malbranchea aurantiaca diphosphate + 1-methyl-4-(3-methylbut-2-enyl)-L-tryptophan 3.2% of the yield with L-tryptophan ?
2.5.1.34 dimethylallyl diphosphate + 5-hydroxy-L-tryptophan
-
 Malbranchea aurantiaca diphosphate + 5-hydroxy-4-(3-methylbut-2-enyl)-L-tryptophan 17.0% of the yield with L-tryptophan ?
2.5.1.34 dimethylallyl diphosphate + 5-methyl-D-tryptophan
-
 Malbranchea aurantiaca diphosphate + 4-(3-methylbut-2-enyl)-5-methyl-D-tryptophan 0.5% of the activity with L-tryptophan ?
2.5.1.34 dimethylallyl diphosphate + 5-methyl-DL-tryptophan
-
 Malbranchea aurantiaca diphosphate + 5-methyl-4-(3-methylbut-2-enyl)-DL-tryptophan 16.1% of the yield with L-tryptophan ?
2.5.1.34 dimethylallyl diphosphate + 5-methyl-L-tryptophan
-
 Malbranchea aurantiaca diphosphate + 4-(3-methylbut-2-enyl)-5-methyl-L-tryptophan
-
 ?
2.5.1.34 dimethylallyl diphosphate + 6-methyl-DL-tryptophan
-
 Malbranchea aurantiaca diphosphate + 6-methyl-4-(3-methylbut-2-enyl)-DL-tryptophan 27.0% of the yield with L-tryptophan ?
2.5.1.34 dimethylallyl diphosphate + L-tryptophan
-
 Malbranchea aurantiaca diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan
-
 ?
2.5.1.34 dimethylallyl diphosphate + Nalpha-methyl-L-tryptophan i.e. L-abrine Malbranchea aurantiaca diphosphate + Nalpha-methyl-L-4-(gamma,gamma-dimethylallyl)tryptophan 24.9% of the yield with L-tryptophan ?

Subunits

EC Number Subunits Comment Organism
2.5.1.34 monomer 1 * 52000, calculated, 1 * 51000, SDS-PAGE, His-tagged protein Malbranchea aurantiaca

Synonyms

EC Number Synonyms Comment Organism
2.5.1.34 MaPT
-
 Malbranchea aurantiaca

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.34 30
-
-
 Malbranchea aurantiaca

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.34 0.56
-
 5-hydroxy-L-tryptophan pH 7.4, 30°C Malbranchea aurantiaca
2.5.1.34 1.06
-
 Nalpha-methyl-L-tryptophan pH 7.4, 30°C Malbranchea aurantiaca
2.5.1.34 1.34
-
 L-tryptophan pH 7.4, 30°C Malbranchea aurantiaca
2.5.1.34 1.4
-
 L-tryptophan presence of D-tryptophan, pH 7.4, 30°C Malbranchea aurantiaca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.34 7.4
-
-
 Malbranchea aurantiaca

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.34 0.0404
-
 D-tryptophan pH 7.4, 30°C Malbranchea aurantiaca