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Literature summary extracted from

  • Koski, M.K.; Hieta, R.; Boellner, C.; Kivirikko, K.I.; Myllyharju, J.; Wierenga, R.K.
    The active site of an algal prolyl 4-hydroxylase has a large structural plasticity (2007), J. Biol. Chem., 282, 37112-37123.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.11.2 structures of apoenzyme at 1.93 and 2.90 A resolution, complexed with the competitive inhibitor Zn2+, and with Zn2+ and pyridine 2,4-dicarboxylate at 1.85 A resolution. The structures reveal the double-stranded alpha-helix core fold typical for 2-oxoglutarate dioxygenases. The catalytic site is at the center of an extended shallow groove lined by two flexible loops Chlamydomonas reinhardtii

Protein Variants

EC Number Protein Variants Comment Organism
1.14.11.2 E127A very low catalytic activity Chlamydomonas reinhardtii
1.14.11.2 H245A no catalytic activity Chlamydomonas reinhardtii
1.14.11.2 Q130A almost 2-fold increase in Km for poly(L-Pro) Chlamydomonas reinhardtii
1.14.11.2 R161A no catalytic activity Chlamydomonas reinhardtii
1.14.11.2 R93A no catalytic activity Chlamydomonas reinhardtii
1.14.11.2 S95A no catalytic activity Chlamydomonas reinhardtii
1.14.11.2 W243A Km values similar to wild-type, 10-fold decrease in kcat Chlamydomonas reinhardtii
1.14.11.2 W99A 2.5-fold increase in Km for poly(L-Pro) Chlamydomonas reinhardtii
1.14.11.2 Y134F 6-fold increase in Km for 2-oxoglutarate, decrease in kcat Chlamydomonas reinhardtii
1.14.11.2 Y140A no catalytic activity Chlamydomonas reinhardtii
1.14.11.2 Y168F 3-4-fold increase in Km for 2-oxoglutarate, decrease in kcat Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.2 0.13
-
 2-oxoglutarate mutant W99A Chlamydomonas reinhardtii
1.14.11.2 0.14
-
 2-oxoglutarate mutant W243A Chlamydomonas reinhardtii
1.14.11.2 0.15
-
 2-oxoglutarate mutant Q130A Chlamydomonas reinhardtii
1.14.11.2 0.16
-
 2-oxoglutarate wild-type Chlamydomonas reinhardtii
1.14.11.2 0.25
-
 poly(L-Pro) wild-type Chlamydomonas reinhardtii
1.14.11.2 0.27
-
 poly(L-Pro) mutant Y168F Chlamydomonas reinhardtii
1.14.11.2 0.28
-
 poly(L-Pro) mutant W243A Chlamydomonas reinhardtii
1.14.11.2 0.28
-
 poly(L-Pro) mutant Y134F Chlamydomonas reinhardtii
1.14.11.2 0.46
-
 poly(L-Pro) mutant Q130A Chlamydomonas reinhardtii
1.14.11.2 0.57
-
 2-oxoglutarate mutant Y168F Chlamydomonas reinhardtii
1.14.11.2 0.65
-
 poly(L-Pro) mutant W99A Chlamydomonas reinhardtii
1.14.11.2 1
-
 2-oxoglutarate mutant Y134F Chlamydomonas reinhardtii

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.2 Chlamydomonas reinhardtii
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.2 poly(L-Pro) + 2-oxoglutarate + O2 Mr of poly(L-Pro) is about 5000 Chlamydomonas reinhardtii poly(4-hydroxyproline) + succinate + CO2
-
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Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.11.2 3
-
 poly(L-Pro) mutant Y134F Chlamydomonas reinhardtii
1.14.11.2 6
-
 poly(L-Pro) mutant W243A Chlamydomonas reinhardtii
1.14.11.2 20
-
 poly(L-Pro) mutant W99A Chlamydomonas reinhardtii
1.14.11.2 30
-
 poly(L-Pro) mutant Y168F Chlamydomonas reinhardtii
1.14.11.2 35
-
 poly(L-Pro) mutant Q130A Chlamydomonas reinhardtii
1.14.11.2 70
-
 poly(L-Pro) wild-type Chlamydomonas reinhardtii