Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Obiozo, U.M.; Brondijk, T.H.; White, A.J.; van Boxel, G.; Dafforn, T.R.; White, S.A.; Jackson, J.B.
    Substitution of tyrosine 146 in the dI component of proton-translocating transhydrogenase leads to reversible dissociation of the active dimer into inactive monomers (2007), J. Biol. Chem., 282, 36434-36443.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.1.1.1 expressed in Escherichia coli BL21(DE3) cells Rhodospirillum rubrum

Protein Variants

EC Number Protein Variants Comment Organism
7.1.1.1 Y146A mutation in component dI that binds NADH. dI.Y146A more readily dissociates into monomers than wild-type dI. dI.Y146A monomers bind NADH much more weakly than dimers. dI.Y146A reconstitutes activity to dI-depleted membranes in its dimeric form but not in its monomeric form Rhodospirillum rubrum
7.1.1.1 Y146A the mutant binds NADH much more weakly than the wild type enzyme Rhodospirillum rubrum
7.1.1.1 Y146F mutation in component dI that binds NADH. Wild-type dI and dI.Y146F reconstituted activity to dI-depleted membranes with similar characteristics Rhodospirillum rubrum
7.1.1.1 Y146F the mutant shows wild type NADH binding ability Rhodospirillum rubrum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.1.1 membrane
-
Rhodospirillum rubrum 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.1.1 40300
-
2 * 40300, calculated from amino acid sequence Rhodospirillum rubrum
7.1.1.1 84000
-
gel filtration Rhodospirillum rubrum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.1.1.1 NADPH + NAD+ + H+[side 1] Rhodospirillum rubrum
-
NADP+ + NADH + H+[side 2]
-
?

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.1 Rhodospirillum rubrum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.1 NADPH + NAD+ + H+[side 1]
-
Rhodospirillum rubrum NADP+ + NADH + H+[side 2]
-
?
7.1.1.1 NADPH + oxidized acetyl pyridine adenine dinucleotide + H+[side 1]
-
Rhodospirillum rubrum NADP+ + reduced acetyl pyridine adenine dinucleotide + H+[side 2]
-
r

Subunits

EC Number Subunits Comment Organism
7.1.1.1 dimer 2 * 40300, calculated from amino acid sequence Rhodospirillum rubrum
7.1.1.1 More the protein has three components: dI binds NADH, dIII binds NADP+, and dII spans the membrane. Transhydrogenase is a dimer of two dI-dII-dIII monomers. The two catalytic sites alternate during turnover Rhodospirillum rubrum

Synonyms

EC Number Synonyms Comment Organism
7.1.1.1 proton-translocating transhydrogenase
-
Rhodospirillum rubrum
7.1.1.1 transhydrogenase
-
Rhodospirillum rubrum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
7.1.1.1 -15
-
the enzyme remains stable for more than 3 months at -15°C Rhodospirillum rubrum
7.1.1.1 4
-
the enzyme remains stable for more than 2 days at 4°C Rhodospirillum rubrum