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Literature summary extracted from

  • Toth, J.; Varga, B.; Kovacs, M.; Malnasi-Csizmadia, A.; Vertessy, B.G.
    Kinetic mechanism of human dUTPase, an essential nucleotide pyrophosphatase enzyme (2007), J. Biol. Chem., 282, 33572-33582.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.6.1.23 drug development the nuclear isoform of the enzyme is a target for anticancer chemotherapeutic strategies Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.23 expression of the His-tagged nuclear isoform and its F158W mutant Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.23 F158W site-directed mutagenesis, inactive mutant Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.23 alpha,beta-imido-dUTP
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.23 additional information
-
 additional information detailed pre-steady-state and steady-state kinetic analysis, quantitative kinetic model of the human dUTPase catalytic cycle, overview Homo sapiens
3.6.1.23 0.0036
-
 dUTP pH 7.4, 20°C, recombinant wild-type enzyme Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.23 nucleus nuclear isozyme Homo sapiens 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.23 Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.23 dUTP + H2O Homo sapiens dUTPase is essential in controlling relative cellular levels of dTTP/dUTP, both of which can be incorporated into DNA dUMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.23 Homo sapiens P33316
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.23 dUTP + H2O dUTPase is essential in controlling relative cellular levels of dTTP/dUTP, both of which can be incorporated into DNA Homo sapiens dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O substrate synthesis, overview, the enzymatic cycle is limited by a single rate-limiting step that occurs before or is concomitant with proton release Homo sapiens dUMP + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.23 dUTPase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.23 20
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.23 6.58
-
 dUTP pH 7.4, 20°C, recombinant wild-type enzyme Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.23 7.4
-
 assay at Homo sapiens