Literature summary extracted from

Neiers, F.; Sonkaria, S.; Olry, A.; Boschi-Muller, S.; Branlant, G.
Characterization of the amino acids from Neisseria meningitidis methionine sulfoxide reductase B involved in the chemical catalysis and substrate specificity of the reductase step (2007), J. Biol. Chem., 282, 32397-32405.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.8.4.12	expression in Escherichia coli	Neisseria meningitidis

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.8.4.12	additional information	-	additional information	reductase step kinetic parameters of the wild-type and mutated MsrB	Neisseria meningitidis

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.8.4.12	Neisseria meningitidis	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.8.4.12	-	Neisseria meningitidis

Reaction

EC Number	Reaction	Comment	Organism	Reaction ID
1.8.4.12	peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (S)-S-oxide + thioredoxin	formation of the MsrB substrate complex leads to an activation of the catalytic Cys-117 characterized by a decreased pKapp of about 2.7 pH units. The catalytic active MsrB form is the Cys117-/His103+ species with a pKapp of 6.6 and 8.3, respectively. His103 and to a lesser extent His100, Asn119, and Thr26 (via a water molecule) participate in the stabilization of the polarized form of the sulfoxide function and of the transition state. Trp65 is essential for the catalytic efficiency of the reductase step by optimizing the position of the substrate in the active site	Neisseria meningitidis	a

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.8.4.12	acetyl-L-methionine-(R)-S-oxide-NHMe + thioredoxin	-	Neisseria meningitidis	?	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
1.8.4.12	methionine sulfoxide reductase B	-	Neisseria meningitidis
1.8.4.12	MsrB	-	Neisseria meningitidis

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.8.4.12	additional information	-	additional information	reductase step kinetic parameters of the wild-type and mutated MsrB	Neisseria meningitidis

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Release 2023.1 (January 2023)