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Literature summary extracted from

  • Gavilano, L.B.; Coleman, N.P.; Bowen, S.W.; Siminszky, B.
    Functional analysis of nicotine demethylase genes reveals insights into the evolution of modern tobacco (2007), J. Biol. Chem., 282, 249-256.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.B11 C330W isoform CYP82E3 variant, active Nicotiana tomentosiformis
1.14.14.B11 K269N isoform CYP82E3 variant, no catalytic activity Nicotiana tomentosiformis
1.14.14.B11 K269N/I274Null isoform CYP82E3 variant, no catalytic activity Nicotiana tomentosiformis
1.14.14.B11 K269N/I274Null/T279A isoform CYP82E3 variant, no catalytic activity Nicotiana tomentosiformis
1.14.14.B11 additional information wild-type isoform CYP82E3 shares 95% predicted amino acid sequence identity with isoform CYP82E4 but conferrs no activity when expressed in yeast or tobacco. Series of domain-swapping experiments between isoforms CYP82E3 and CYP82E4 to isentify regions and amino acids required for catalytic activity Nicotiana tabacum
1.14.14.B11 additional information wild-type isoform CYP82E3 shares 95% predicted amino acid sequence identity with isoform CYP82E4 but confers no activity when expressed in yeast or tobacco. Series of domain-swapping experiments between isoforms CYP82E3 and CYP82E4 to identify regions and amino acids required for catalytic activity Nicotiana tomentosiformis
1.14.14.B11 additional information wild-type isoform CYP82E3 shares 95% predicted amino acid sequence identity with isoform CYP82E4 but confers no activity when expressed in yeast or tobacco. Series of domain-swapping experiments between isoforms CYP82E3 and CYP82E4 to isentify regions and amino acids required for catalytic activity Nicotiana tomentosiformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.B11 0.0091
-
 Nicotine 23°C, pH 7.1 Nicotiana tomentosiformis
1.14.14.B11 0.0481
-
 Nicotine 23°C, pH 7.1 Nicotiana tomentosiformis

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.B11 Nicotiana tabacum
-
 isoform CYP82E3
-
1.14.14.B11 Nicotiana tomentosiformis A1YJE2 isoform CYP82E3
-
1.14.14.B11 Nicotiana tomentosiformis A1YJE3 isoform CYP82E4
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.B11 leaf high expression in green leaf Nicotiana tomentosiformis
-
1.14.14.B11 leaf high induced during leaf senescence Nicotiana tomentosiformis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.B11 nicotine + [reduced NADPH-hemoprotein reductase] + O2
-
 Nicotiana tomentosiformis nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?