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Literature summary extracted from

  • Hung, R.J.; Chien, H.S.; Lin, R.Z.; Lin, C.T.; Vatsyayan, J.; Peng, H.L.; Chang, H.Y.
    Comparative analysis of two UDP-glucose dehydrogenases in Pseudomonas aeruginosa PAO1 (2007), J. Biol. Chem., 282, 17738-17748.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.22 expression in Escherichia coli Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.22 additional information mutant lacking PA2022 activity and double mutant lacking PA2022 and isoform PA3559 activity are more susceptible to chloramphenicol, ceffotaxime, and ampicillin Pseudomonas aeruginosa
1.1.1.22 additional information mutant lacking PA3559 activity shows reduced resistance to polymyxin B Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.22 Ca2+ 84% residual activity at 1 mM Pseudomonas aeruginosa
1.1.1.22 EDTA 83% residual activity at 1 mM Pseudomonas aeruginosa
1.1.1.22 Mn2+ 84% residual activity at 1 mM Pseudomonas aeruginosa
1.1.1.22 NADH product inhibition, 26% residual activity at 0.05 mM; product inhibition, 70% residual activity at 0.05 mM Pseudomonas aeruginosa
1.1.1.22 UDP-glucuronic acid product inhibition, 44% residual activity at 1 mM; product inhibition, 79% residual activity at 1 mM Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.22 0.12
-
 UDP-glucose pH 7.5, 22°C Pseudomonas aeruginosa
1.1.1.22 0.4
-
 UDP-glucose pH 7.5, 22°C Pseudomonas aeruginosa
1.1.1.22 0.47
-
 NAD+ pH 7.5, 22°C Pseudomonas aeruginosa
1.1.1.22 1.99
-
 NAD+ pH 7.5, 22°C Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.22 54500
-
 54500, calculated Pseudomonas aeruginosa
1.1.1.22 57400
-
 57400, calculated Pseudomonas aeruginosa
1.1.1.22 104000
-
 gel filtration Pseudomonas aeruginosa
1.1.1.22 117000
-
 gel filtration Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.22 Pseudomonas aeruginosa O86422 isoform PA2022, constitutively expressed
-
1.1.1.22 Pseudomonas aeruginosa Q9HY58 isoform PA3559, expressed primarily in low concentrations of Mg2+
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.22 TDP-glucose + NAD+ + H2O 38.5% of the rate with UDP-glucose Pseudomonas aeruginosa TDP-glucuronate + NADH
-
 ?
1.1.1.22 UDP-galactose + NAD+ + H2O 11.9% of the rate with UDP-glucose Pseudomonas aeruginosa UDP-galacturonate + NADH
-
 ?
1.1.1.22 UDP-galactose + NAD+ + H2O 6.4% of the rate with UDP-glucose Pseudomonas aeruginosa UDP-galacturonate + NADH
-
 ?
1.1.1.22 UDP-glucose + 2 NAD+ + H2O
-
 Pseudomonas aeruginosa UDP-glucuronate + 2 NADH + 2 H+
-
 ?
1.1.1.22 UDP-N-acetylglucosamine + NAD+ + H2O 35% of the rate with UDP-glucose Pseudomonas aeruginosa ? + NADH
-
 ?
1.1.1.22 UDP-N-acetylglucosamine + NAD+ + H2O 6.3% of the rate with UDP-glucose Pseudomonas aeruginosa ? + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.22 dimer 54500, calculated Pseudomonas aeruginosa
1.1.1.22 dimer 57400, calculated Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
1.1.1.22 PA2022
-
 Pseudomonas aeruginosa
1.1.1.22 PA3559
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.22 0.24
-
 UDP-glucose pH 7.5, 22°C Pseudomonas aeruginosa
1.1.1.22 0.37
-
 NAD+ pH 7.5, 22°C Pseudomonas aeruginosa
1.1.1.22 0.81
-
 UDP-glucose pH 7.5, 22°C Pseudomonas aeruginosa
1.1.1.22 2.35
-
 NAD+ pH 7.5, 22°C Pseudomonas aeruginosa