EC Number | Cloned (Comment) | Organism |
---|---|---|
1.17.1.8 | expressed in Escherichia coli XL-1 Blue cells | Thermotoga maritima |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.17.1.8 | 2,3-dihydrodipicolinate | the enzyme is inhibited by high concentrations of 2,3-dihydrodipicolinate | Thermotoga maritima | |
1.17.1.8 | NADH | - |
Thermotoga maritima |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.17.1.8 | 0.0006 | - |
NADPH | apparent value, at 30°C | Thermotoga maritima | |
1.17.1.8 | 0.0018 | - |
NADH | apparent value, at 45°C | Thermotoga maritima | |
1.17.1.8 | 0.0021 | - |
NADPH | apparent value, at 45°C | Thermotoga maritima | |
1.17.1.8 | 0.0025 | - |
NADH | apparent value, at 30°C | Thermotoga maritima | |
1.17.1.8 | 0.0076 | - |
2,3-dihydrodipicolinate | apparent value, at 30°C | Thermotoga maritima | |
1.17.1.8 | 0.013 | - |
2,3-dihydrodipicolinate | apparent value, at 45°C | Thermotoga maritima |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.17.1.8 | Thermotoga maritima | Q9X1K8 | - |
- |
1.17.1.8 | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | Q9X1K8 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.17.1.8 | His-Trap column chromatography | Thermotoga maritima |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.1.8 | 2,3-dihydrodipicolinate + NAD(P)H | - |
Thermotoga maritima | 2,3,4,5-tetrahydrodipicolinate + NAD(P)+ | - |
? | |
1.17.1.8 | 2,3-dihydrodipicolinate + NAD(P)H | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | 2,3,4,5-tetrahydrodipicolinate + NAD(P)+ | - |
? | |
1.17.1.8 | 2,3-dihydrodipicolinate + NADH + H+ | - |
Thermotoga maritima | 2,3,4,5-tetrahydrodipicolinate + NAD+ + H2O | - |
r | |
1.17.1.8 | 2,3-dihydrodipicolinate + NADH + H+ | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | 2,3,4,5-tetrahydrodipicolinate + NAD+ + H2O | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.17.1.8 | tetramer | x-ray crystallography | Thermotoga maritima |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.17.1.8 | DHDPR | - |
Thermotoga maritima |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.17.1.8 | 80 | 95 | the enzyme is stable for up to 48 h at 80°C, the melting temperature of DHDPR is at 95.7°C | Thermotoga maritima |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.17.1.8 | 0.093 | - |
NADH | apparent value, at 30°C | Thermotoga maritima | |
1.17.1.8 | 0.49 | - |
NADH | apparent value, at 45°C | Thermotoga maritima | |
1.17.1.8 | 2.6 | - |
NADPH | apparent value, at 30°C | Thermotoga maritima | |
1.17.1.8 | 8.1 | - |
2,3-dihydrodipicolinate | apparent value, at 30°C | Thermotoga maritima | |
1.17.1.8 | 17 | - |
NADPH | apparent value, at 45°C | Thermotoga maritima | |
1.17.1.8 | 19 | - |
2,3-dihydrodipicolinate | apparent value, at 45°C | Thermotoga maritima |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.17.1.8 | NADH | the enzyme has a greater affinity for NADPH than for NADH | Thermotoga maritima | |
1.17.1.8 | NADPH | the enzyme has a greater affinity for NADPH than for NADH | Thermotoga maritima |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.17.1.8 | 0.0051 | - |
NADH | at 30°C | Thermotoga maritima | |
1.17.1.8 | 0.0081 | - |
NADH | at 45°C | Thermotoga maritima |