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Literature summary extracted from

  • Georgi, T.; Engels, V.; Wendisch, V.F.
    Regulation of L-lactate utilization by the FadR-type regulator LldR of Corynebacterium glutamicum (2008), J. Bacteriol., 190, 963-971.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.1.5.B3 additional information overexpression of the regulator gene lldR results in strongly reduced NCgl2816-lldD mRNA levels and strongly reduced LldD activity, and as a consequence, in a severe growth defect was observed in cells grown on L-lactate as the sole carbon and energy source, but not in cells grown on glucose, ribose, or acetate. Deletion of lldR has no effect on growth on these carbon sources but results in high NCgl2816-lldD mRNA levels and high LldD activity in the presence and absence of L-lactate, overview Corynebacterium glutamicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.5.B3 additional information the enzyme expression is supressed/regulated by the FadR-type regulator LldR, encoded by NCgl2814, binds to the upstream region of NCgl2816-lldD, L-lactate prevents binding of LldRHis to the NCgl2816-lldD promoter region Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.5.B3 L-lactate + oxidized 2,6-dichlorophenolindophenol Corynebacterium glutamicum
-
 pyruvate + reduced 2,6-dichlorophenolindophenol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.B3 Corynebacterium glutamicum
-
 gene lldD
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.5.B3 additional information
-
-
 Corynebacterium glutamicum
1.1.5.B3 0.14
-
 wild-type strain, cell extract Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.B3 L-lactate + oxidized 2,6-dichlorophenolindophenol
-
 Corynebacterium glutamicum pyruvate + reduced 2,6-dichlorophenolindophenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.5.B3 LldD
-
 Corynebacterium glutamicum
1.1.5.B3 quinone-dependent L-lactate dehydrogenase
-
 Corynebacterium glutamicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.5.B3 30
-
 assay at Corynebacterium glutamicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.B3 7.5
-
 assay at Corynebacterium glutamicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.B3 2,6-dichlorophenolindophenol
-
 Corynebacterium glutamicum
1.1.5.B3 quinone dependent on Corynebacterium glutamicum