EC Number | Application | Comment | Organism |
---|---|---|---|
1.2.1.61 | additional information | HapE shows 45% sequence identity with CymC, a p-cumic aldehyde dehydrogenase, from Pseudomonas putida | Pseudomonas fluorescens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.84 | gne hapA, encoded in the hapCDEFGHIBA gene cluster, DNA and amino acid sequence determination and analysis | Pseudomonas fluorescens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.61 | 52400 | - |
- |
Pseudomonas fluorescens |
1.14.13.84 | 71957 | - |
x * 71957, sequence calculation | Pseudomonas fluorescens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.84 | 4-hydroxyacetophenone + NADPH + H+ + O2 | Pseudomonas fluorescens | the enzyme catalyzes the first committed step in th 4-hydroxyacetophenone catabolism, pathway overview | 4-hydroxyphenyl acetate + NADP+ + H2O | - |
? | |
1.14.13.84 | 4-hydroxyacetophenone + NADPH + H+ + O2 | Pseudomonas fluorescens ACB | the enzyme catalyzes the first committed step in th 4-hydroxyacetophenone catabolism, pathway overview | 4-hydroxyphenyl acetate + NADP+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.61 | Pseudomonas fluorescens | - |
- |
- |
1.2.1.61 | Pseudomonas fluorescens ACB | - |
- |
- |
1.14.13.84 | Pseudomonas fluorescens | Q93TJ5 | gene hapA, formerly hapE, encoded in the gene cluster hapCDEFGHIBA | - |
1.14.13.84 | Pseudomonas fluorescens ACB | Q93TJ5 | gene hapA, formerly hapE, encoded in the gene cluster hapCDEFGHIBA | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.61 | 4-hydroxymuconic semialdehyde + NAD+ + H2O | - |
Pseudomonas fluorescens | maleylacetate + NADH + H+ | - |
? | |
1.2.1.61 | 4-hydroxymuconic semialdehyde + NAD+ + H2O | - |
Pseudomonas fluorescens ACB | maleylacetate + NADH + H+ | - |
? | |
1.14.13.84 | 4-hydroxyacetophenone + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | 4-hydroxyphenyl acetate + NADP+ + H2O | - |
? | |
1.14.13.84 | 4-hydroxyacetophenone + NADPH + H+ + O2 | the enzyme catalyzes the first committed step in th 4-hydroxyacetophenone catabolism, pathway overview | Pseudomonas fluorescens | 4-hydroxyphenyl acetate + NADP+ + H2O | - |
? | |
1.14.13.84 | 4-hydroxyacetophenone + NADPH + H+ + O2 | - |
Pseudomonas fluorescens ACB | 4-hydroxyphenyl acetate + NADP+ + H2O | - |
? | |
1.14.13.84 | 4-hydroxyacetophenone + NADPH + H+ + O2 | the enzyme catalyzes the first committed step in th 4-hydroxyacetophenone catabolism, pathway overview | Pseudomonas fluorescens ACB | 4-hydroxyphenyl acetate + NADP+ + H2O | - |
? | |
1.14.13.84 | additional information | the enzyme is active with a wide range of aromatic and aliphatic ketones | Pseudomonas fluorescens | ? | - |
? | |
1.14.13.84 | additional information | the enzyme is active with a wide range of aromatic and aliphatic ketones | Pseudomonas fluorescens ACB | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.13.84 | ? | x * 71957, sequence calculation | Pseudomonas fluorescens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.61 | 4-hydroxymuconic semialdehyde dehydrogenase | - |
Pseudomonas fluorescens |
1.2.1.61 | hAPE | - |
Pseudomonas fluorescens |
1.14.13.84 | hAPA | - |
Pseudomonas fluorescens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.13.84 | 25 | - |
assay at | Pseudomonas fluorescens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.84 | 7 | - |
assay at | Pseudomonas fluorescens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.61 | NAD+ | - |
Pseudomonas fluorescens | |
1.14.13.84 | NADPH | - |
Pseudomonas fluorescens |