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Literature summary extracted from

  • Chen, Y.; Zhang, X.; Manias, D.; Yeo, H.; Dunny, G.M.; Christie, P.J.
    Enterococcus faecalis PcfC, a spatially localized substrate receptor for type IV secretion of the pCF10 transfer intermediate (2008), J. Bacteriol., 190, 3632-3645.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.4.2.8 nine pcfF expression plasmids are constructed, different in the combination of the promotor, Pnis, P23, Ptac, PT7, the affinity tag, His6, GST, and the cloned PcfC fragment, complete cDNA, deltaN103 or mutant K156T Enterococcus faecalis

Protein Variants

EC Number Protein Variants Comment Organism
7.4.2.8 deltaN103 pcfF mutant lacking codon 1 to 103 Enterococcus faecalis
7.4.2.8 K156T pcfF mutant Enterococcus faecalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.4.2.8 membrane membrane-bound Enterococcus faecalis 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.4.2.8 60000
-
 His6-PcfCdeltaN103, determined by size exclusion chromatography Enterococcus faecalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.4.2.8 ATP + H2O Enterococcus faecalis in addition the protein binds DNA and interacts with PcfF and PcfG ADP + phosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
7.4.2.8 Enterococcus faecalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.4.2.8 by affinity chromatography, using Talon, Co2+, resin and glutathione-Sepharose beads, and by gel filtration Enterococcus faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.4.2.8 ATP + H2O in addition the protein binds DNA and interacts with PcfF and PcfG Enterococcus faecalis ADP + phosphate
-
 ir

Synonyms

EC Number Synonyms Comment Organism
7.4.2.8 PcfC
-
 Enterococcus faecalis
7.4.2.8 protein-secreting ATPase
-
 Enterococcus faecalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7.4.2.8 22
-
 ATP binding assay at room temperature Enterococcus faecalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4.2.8 8
-
 ATP binding assay Enterococcus faecalis