EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.4.11 | expression inm Escherichia coli, recombinant proteins corresponding to MsrABTk and the individual domains (MsrATk and MsrBTk) are produced | Thermococcus kodakarensis |
1.8.4.12 | expression inm Escherichia coli, recombinant proteins corresponding to MsrABTk and the individual domains (MsrATk and MsrBTk) are produced | Thermococcus kodakarensis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.4.11 | 15 | - |
dithiothreitol | MsrA domain of MsrABTk, in the presence of 80 mM L-methionine (S)-sulfoxide | Thermococcus kodakarensis | |
1.8.4.11 | 19 | - |
L-methionine (S)-sulfoxide | MsrA domain of MsrABTk, in the presence of 100 mM dithiothreiol | Thermococcus kodakarensis | |
1.8.4.12 | 2 | - |
dithiothreitol | MsrB domain of MsrABTk, in the presence of 80 mM L-methionine (S)-sulfoxide | Thermococcus kodakarensis | |
1.8.4.12 | 67 | - |
L-methionine (R)-sulfoxide | MsrB domain of MsrABTk, in the presence of 20 mM dithiothreiol | Thermococcus kodakarensis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.4.11 | Thermococcus kodakarensis | - |
- |
- |
1.8.4.12 | Thermococcus kodakarensis | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.8.4.11 | additional information | presence o MsrABTk is greater in Thermococcus kodakaraensis cells grown at suboptimal temperatures (60 to 70°C) and could not be detected at 80 to 90°C. The amount of intracellular MsrABTk protein increases with exposure to higher dissolved oxygen levels, but only at suboptimal growth temperatures | Thermococcus kodakarensis | - |
1.8.4.12 | additional information | presence of MsrABTk is greater in Thermococcus kodakaraensis cells grown at suboptimal temperatures (60 to 70°C) and could not be detected at 80 to 90°C. The amount of intracellular MsrABTk protein increases with exposure to higher dissolved oxygen levels, but only at suboptimal growth temperatures | Thermococcus kodakarensis | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.4.11 | dabsyl-L-methionine-(S)-S-oxide + dithiothreitol | - |
Thermococcus kodakarensis | dabsyl-L-methionine + dithiothreitol disulfide + H2O | - |
? | |
1.8.4.11 | L-methionine (S)-sulfoxide + 2 dithiothreitol | the MsrA-domain of MsrABTk is strictly specific for the reduction of L-methionine (S)-sulfoxide | Thermococcus kodakarensis | L-methionine + dithiothreitol disulfide + H2O | - |
? | |
1.8.4.12 | dabsyl-L-methionine-(R)-S-oxide + dithiothreitol | - |
Thermococcus kodakarensis | dabsyl-L-methionine + dithiothreitol disulfide + H2O | - |
? | |
1.8.4.12 | L-methionine (R)-sulfoxide + dithiothreitol | the MsrB-domain of MsrABTk is strictly specific for the reduction of L-methionine (R)-sulfoxide | Thermococcus kodakarensis | L-methionine + dithiothreitol disulfide | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.4.11 | MsrABTk | bifunctional enzyme (EC 1.8.1.11/1.8.1.12) | Thermococcus kodakarensis |
1.8.4.12 | MsrABTk | bifunctional enzyme (EC 1.8.1.11/1.8.1.12) | Thermococcus kodakarensis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.8.4.11 | 60 | - |
MsrA domain of MsrABTk, substrate: dabsyl-L-methionine-(S)-S-oxide | Thermococcus kodakarensis |
1.8.4.12 | 30 | - |
MsrB domain of MsrABTk | Thermococcus kodakarensis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.8.4.11 | 80 | - |
half-life of MsrABTk is 30 min. Half-life of the MsrA domain within the MsrABTk protein is 27 min. The recombinant MsrA protein shows a half-life of less than 1 min. The MsrA domain is stabilized through its physical interaction with the more thermostable MsrB domain | Thermococcus kodakarensis |
1.8.4.11 | 85 | - |
75% decrease in activity after 2.5 min, MsrABTk | Thermococcus kodakarensis |
1.8.4.12 | 80 | - |
half-life of MsrABTk is 30 min, half-life of MsrB domain is 31 min | Thermococcus kodakarensis |
1.8.4.12 | 85 | - |
75% decrease in activity after 2.5 min, MsrABTk | Thermococcus kodakarensis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.8.4.11 | 7.5 | 8 | optimum pH of the MsrA domain | Thermococcus kodakarensis |
1.8.4.12 | 6 | 7 | optimum pH of the MsrB domain | Thermococcus kodakarensis |