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Literature summary extracted from

  • Thulasiram, H.V.; Erickson, H.K.; Poulter, C.D.
    A common mechanism for branching, cyclopropanation, and cyclobutanation reactions in the isoprenoid biosynthetic pathway (2008), J. Am. Chem. Soc., 130, 1966-1971.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.67 a c98f chimera is constructed by replacing the first 98 residues in Artemisia tridentata subsp. spiciformis farnesyl diphosphate synthase with the corresponding sequence from chrysanthemyl diphosphate synthase, the enzymes are cloned in Escherichia coli hosts Artemisia spiciformis

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.69 additional information chimera c98f, constructed by replacing the first 98 residues in Artemisia tridentata ssp. spiciformis farnesyl diphosphate synthase with the corresponding sequence from Artemisia tridentata chrysanthemyl diphosphate synthase is able to produce a 6:5 mixture of (R)-lavandulol and (R)-maconelliol and a small amount of planococcyl alcohol in presence of alkaline phosphatase. Chimeric proteins constructed from farnesyl diphosphate synthase, which catalyzes chain elongation, and chrysanthemyl diphosphate synthase, which catalyzes cyclopropanation, catalyze all four of the known isoprenoid coupling reactions to give a mixture of geranyl diphosphate by chain elongation, chrysanthemyl diphosphate by cyclopropanation, lavandulyl diphosphate by branching, and maconelliyl and planococcyl diphosphate by cyclobutanation Artemisia tridentata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.67 MgCl2 10 mM are included in assay medium Artemisia spiciformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.67 6 dimethylallyl diphosphate Artemisia spiciformis
-
 (R)-lavandulyl diphosphate + (R)-maconelliyl diphosphate + (1R,3R)-chyrsanthemyl diphosphate + 3 diphosphate non-head-to-tail alcohols after incubation of dimethylallyl diphosphate with CPP synthase followed by hydrolysis with alkaline phosphatase ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.67 Artemisia spiciformis Q7XYS8
-
-
2.5.1.67 Artemisia spiciformis Q7XYS8 subsp. spiciformis
-
2.5.1.69 Artemisia tridentata
-
 ssp. spiciformis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.67 2 dimethylallyl diphosphate
-
 Artemisia spiciformis diphosphate + chrysanthemyl diphosphate
-
 ?
2.5.1.67 6 dimethylallyl diphosphate
-
 Artemisia spiciformis (R)-lavandulyl diphosphate + (R)-maconelliyl diphosphate + (1R,3R)-chyrsanthemyl diphosphate + 3 diphosphate non-head-to-tail alcohols after incubation of dimethylallyl diphosphate with CPP synthase followed by hydrolysis with alkaline phosphatase ?
2.5.1.69 2 dimethylallyl diphosphate
-
 Artemisia tridentata (R)-lavandulol + (R)-maconelliol + diphosphate in presence of alkaline phosphatase, chimera c98f produces a 6:5 mixture of (R)-lavandulol and (R)-maconelliol and a small amount of planococcyl alcohol ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.67 chrysanthemyl diphosphate synthase
-
 Artemisia spiciformis
2.5.1.67 CPP synthase
-
 Artemisia spiciformis
2.5.1.67 CPPase
-
 Artemisia spiciformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.67 30
-
 assay at Artemisia spiciformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.67 7.6
-
 assay at Artemisia spiciformis

General Information

EC Number General Information Comment Organism
2.5.1.67 physiological function biosynthesis of isoprenoid compounds Artemisia spiciformis