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Literature summary extracted from

  • Zhu, X.; Robinson, D.A.; McEwan, A.R.; OHagan, D.; Naismith, J.H.
    Mechanism of enzymatic fluorination in Streptomyces cattleya (2007), J. Am. Chem. Soc., 129, 14597-14604.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.63 mutant enzymes expressed in Escherichia coli C43 (DE3) cells Streptomyces cattleya

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.63
-
 Streptomyces cattleya

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.63 D16A inactive Streptomyces cattleya
2.5.1.63 D16N 3% activity compared to the wild type enzyme Streptomyces cattleya
2.5.1.63 D16S inactive Streptomyces cattleya
2.5.1.63 F156A 3% activity compared to the wild type enzyme Streptomyces cattleya
2.5.1.63 F156V 25% activity compared to the wild type enzyme Streptomyces cattleya
2.5.1.63 S158A 38% activity compared to the wild type enzyme Streptomyces cattleya
2.5.1.63 S158G 8% activity compared to the wild type enzyme Streptomyces cattleya
2.5.1.63 T80A 15% activity compared to the wild type enzyme Streptomyces cattleya
2.5.1.63 T80S 95% activity compared to the wild type enzyme Streptomyces cattleya

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.63 0.0008
-
 S-adenosyl-L-methionine mutant enzyme S158G, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
2.5.1.63 0.003
-
 S-adenosyl-L-methionine mutant enzyme T80A, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
2.5.1.63 0.0047
-
 S-adenosyl-L-methionine mutant enzyme T80S, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
2.5.1.63 0.0065
-
 S-adenosyl-L-methionine wild type enzyme, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
2.5.1.63 0.0092
-
 S-adenosyl-L-methionine mutant enzyme S158A, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
2.5.1.63 1.36
-
 fluoride mutant enzyme S158G, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
2.5.1.63 5.4
-
 fluoride mutant enzyme S158A, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
2.5.1.63 10.2
-
 fluoride wild type enzyme, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
2.5.1.63 18.4
-
 fluoride mutant enzyme T80S, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
2.5.1.63 36.8
-
 fluoride mutant enzyme T80A, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.63 Streptomyces cattleya
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.63 S-adenosyl-L-methionine + fluoride
-
 Streptomyces cattleya 5'-deoxy-5'-fluoroadenosine + L-methionine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.63 5'-fluoro-5'-deoxy adenosine synthetase
-
 Streptomyces cattleya
2.5.1.63 FDAS
-
 Streptomyces cattleya

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.63 0.000012
-
 fluoride mutant enzyme S158G, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
2.5.1.63 0.000013
-
 fluoride mutant enzyme S158G, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
2.5.1.63 0.000067
-
 fluoride mutant enzyme T80A, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
2.5.1.63 0.000083
-
 S-adenosyl-L-methionine mutant enzyme T80A, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
2.5.1.63 0.0001
-
 fluoride mutant enzyme S158A, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
2.5.1.63 0.00015
-
 S-adenosyl-L-methionine mutant enzyme S158A, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
2.5.1.63 0.001
-
 S-adenosyl-L-methionine mutant enzyme T80S, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
2.5.1.63 0.001
-
 fluoride mutant enzyme T80S, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
2.5.1.63 0.001
-
 fluoride wild type enzyme, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya
2.5.1.63 0.0012
-
 S-adenosyl-L-methionine wild type enzyme, in 20 mM sodium phosphate buffer (pH 7.8), at 25°C Streptomyces cattleya