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Literature summary extracted from

  • Segovia-Bravo, K.A.; Jaren-Galan, M.; Garcia-Garcia, P.; Garrido-Fernandez, A.
    Characterization of polyphenol oxidase from the Manzanilla cultivar (Olea europaea pomiformis) and prevention of browning reactions in bruised olive fruits (2007), J. Agric. Food Chem., 55, 6515-6520.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 additional information the inhibition of enzyme activity as a function of pH, temperature, and ascorbic acid is studied. The enzymatic activity increases with temperature (within the range studied) and is completely inhibited at pH values below 3.0 regardless of temperature. In alkaline conditions, the inhibitory pH level depends on temperature, at 8°C it is observed at pH above 9.0, whereas at 25°C, inhibition occurrs at pH values above 11.0. In any case, the browning reaction does not occur in the presence of ascorbic acid, and, theoretically, prevent browning in bruised olives Olea europaea

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Olea europaea
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manzanilla cultivar (Olea europaea pomiformis)
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 4-methylcatechol + 1/2 O2
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Olea europaea 4-methyl-1,2-benzoquinone + H2O
-
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Synonyms

EC Number Synonyms Comment Organism
1.14.18.1 polyphenol oxidase
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Olea europaea
1.14.18.1 PPO
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Olea europaea

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.14.18.1 additional information
-
the experiment is carried out only under the conditions of temperature (8 and 25°C) and pH (3.0 and 10.0 units) used in the PPO study, conditions at which the enzyme shows no activity or only a reduced proportion of it Olea europaea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 6
-
maximum activity Olea europaea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.18.1 additional information
-
the experiment is carried out only under the conditions of temperature (8 and 25°C) and pH (3.0 and 10.0 units) used in the PPO study, conditions at which the enzyme shows no activity or only a reduced proportion of it Olea europaea