Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zheng, K.; Board, P.G.; Fei, X.; Sun, Y.; Lv, S.; Yan, G.; Liu, J.; Shen, J.; Luo, G.
    A novel selenium-containing glutathione transferase zeta1-1, the activity of which surpasses the level of some native glutathione peroxidases (2008), Int. J. Biochem. Cell Biol., 40, 2090-2097.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.9 expressed in Escherichia coli Homo sapiens
2.5.1.18 expressed in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.9 S14A mutant shows reduced selenium content and strongly reduced glutathione peroxidase activity Homo sapiens
1.11.1.9 S15A mutant shows reduced selenium content and reduced glutathione peroxidase activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.9 5.93
-
 GSH wild type enzyme, in the presence of 0.1 mM H2O2, at 37°C and pH 7.0 Homo sapiens
1.11.1.9 7.96
-
 GSH wild type enzyme, in the presence of 0.2 mM H2O2, at 37°C and pH 7.0 Homo sapiens
1.11.1.9 8.99
-
 H2O2 wild type enzyme, in the presence of 0.25 mM GSH, at 37°C and pH 7.0 Homo sapiens
1.11.1.9 9.87
-
 GSH wild type enzyme, in the presence of 0.5 mM H2O2, at 37°C and pH 7.0 Homo sapiens
1.11.1.9 11.1
-
 GSH wild type enzyme, in the presence of 1 mM H2O2, at 37°C and pH 7.0 Homo sapiens
1.11.1.9 12.6
-
 H2O2 wild type enzyme, in the presence of 0.5 mM GSH, at 37°C and pH 7.0 Homo sapiens
1.11.1.9 15.88
-
 H2O2 wild type enzyme, in the presence of 1 mM GSH, at 37°C and pH 7.0 Homo sapiens
1.11.1.9 19.08
-
 H2O2 wild type enzyme, in the presence of 2 mM GSH, at 37°C and pH 7.0 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.9 selenium selenoenzyme Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.9 additional information Homo sapiens selenium containing glutathione transferase zeta1-1 does also possess glutathione peroxidase activity ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.9 Homo sapiens
-
-
-
2.5.1.18 Homo sapiens O43708
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.9 nickel-agarose column chromatography Homo sapiens
2.5.1.18 nickel-agarose column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.9 H2O2 + GSH
-
 Homo sapiens H2O + GSSG
-
 ?
1.11.1.9 additional information selenium containing glutathione transferase zeta1-1 does also possess glutathione peroxidase activity Homo sapiens ?
-
 ?
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Homo sapiens S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.9 GPX
-
 Homo sapiens
1.11.1.9 Se-hGSTZ1-1
-
 Homo sapiens
1.11.1.9 selenium containing glutathione transferase zeta1-1
-
 Homo sapiens
2.5.1.18 glutathione transferase zeta1-1
-
 Homo sapiens
2.5.1.18 GSTZ1-1
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.9 221.7
-
 GSH wild type enzyme, in the presence of 0.1 mM H2O2, at 37°C and pH 7.0 Homo sapiens
1.11.1.9 293.3
-
 GSH wild type enzyme, in the presence of 0.2 mM H2O2, at 37°C and pH 7.0 Homo sapiens
1.11.1.9 316.7
-
 H2O2 wild type enzyme, in the presence of 0.25 mM GSH, at 37°C and pH 7.0 Homo sapiens
1.11.1.9 361.7
-
 GSH wild type enzyme, in the presence of 0.5 mM H2O2, at 37°C and pH 7.0 Homo sapiens
1.11.1.9 408.3
-
 GSH wild type enzyme, in the presence of 1 mM H2O2, at 37°C and pH 7.0 Homo sapiens
1.11.1.9 445
-
 H2O2 wild type enzyme, in the presence of 0.5 mM GSH, at 37°C and pH 7.0 Homo sapiens
1.11.1.9 560
-
 H2O2 wild type enzyme, in the presence of 1 mM GSH, at 37°C and pH 7.0 Homo sapiens
1.11.1.9 670
-
 H2O2 wild type enzyme, in the presence of 2 mM GSH, at 37°C and pH 7.0 Homo sapiens