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Literature summary extracted from

  • Gerken, T.A.; Hagen, K.G.; Jamison, O.
    Conservation of peptide acceptor preferences between Drosophila and mammalian polypeptide-GalNAc transferase orthologue pairs (2008), Glycobiology, 18, 861-870.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.41 expression in COS7 cells as truncated version from pIMKF4 SV40 Drosophila melanogaster

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.41 Mn2+ 10 mM MnCl2 Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide Bos taurus mucin-type O-linked glycosylation of serine or threonine residues UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide Homo sapiens mucin-type O-linked glycosylation of serine or threonine residues UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide Drosophila melanogaster mucin-type O-linked glycosylation of serine or threonine residues UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.41 Bos taurus Q07537 ppGalNAc T1
-
2.4.1.41 Drosophila melanogaster Q6WV17 PGANT5; PGANT5 is the orthologue of mammalian ppGalNAc T1
-
2.4.1.41 Drosophila melanogaster Q6WV19 PGANT2; PGANT2 is the orthologue of mammalian ppGalNAc T2
-
2.4.1.41 Homo sapiens Q10471 ppGalNAc T2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.41 UDP-GalNAc + GAGA(X)3T(X)3AGAGK
-
 Drosophila melanogaster GAGA(X)3(GalNAc)T(X)3AGAGK + UDP
-
 ?
2.4.1.41 UDP-GalNAc + GAGA(X)3T(X)3AGAGK
-
 Bos taurus UDP + GAGA(X)3(GalNAc)T(X)3AGAGK
-
 ?
2.4.1.41 UDP-GalNAc + GAGA(X)3T(X)3AGAGK
-
 Homo sapiens UDP + GAGA(X)3(GalNAc)T(X)3AGAGK
-
 ?
2.4.1.41 UDP-GalNAc + GAGA(X)5T(X)5AGAGK transferase activity relatively insensitive to the nature of amino acids 4 and 5 residues away from site of glycosylation, high correlation between peptide preferences of PGANT5 and mammalian orthologue ppGalNAc T1 with r(square) = 0.67, the hydrophobic residue proline is preferred at the -1, +1, and +3 positions, the hydrophobic residues valine and tyrosin are moderately preferred at the -1 and +3 positions, the hydrophobic residue methionine is disfavoured at the -1 position, low hydrophilic preference at the -1 position, strong glutamate/aspartate preference at the +1 position, higher preference of glutamate at +1 and +2 positions than mammalian orthologue ppGalNAc T1 Drosophila melanogaster GAGA(X)5(GalNAc)T(X)5AGAGK + UDP
-
 ?
2.4.1.41 UDP-GalNAc + GAGA(X)5T(X)5AGAGK transferase activity relatively insensitive to the nature of amino acids 4 and 5 residues away from site of glycosylation, very high correlation between peptide preferences of PGANT2 and mammalian orthologue ppGalNAc T2 with r(square) = 0.92 Drosophila melanogaster GAGA(X)5(GalNAc)T(X)5AGAGK + UDP
-
 ?
2.4.1.41 UDP-GalNAc + GAGA(X)5T(X)5AGAGK very high correlation between peptide preferences of ppGalNAc T2 and fly orthologue PGANT2 with r(square) = 0.92 Homo sapiens UDP + GAGA(X)5(GalNAc)T(X)5AGAGK
-
 ?
2.4.1.41 UDP-GalNAc + GAGA(X)5T(X)5AGAGK very high correlation between peptide preferences of ppGalNAc T2 and fly orthologue PGANT2 with r(square) = 0.92, the hydrophobic residue proline is preferred at the -1, +1, and +3 positions, the hydrophobic residues valine and tyrosin are moderately preferred at the -1 and +3 positions, the hydrophobic residue methionine is disfavoured at the -1 position, low hydrophilic preference at the -1 position, strong glutamate/aspartate preference at the +1 position, lower preference of glutamate at +1 and +2 positions than fly orthologue PGANT5 Bos taurus UDP + GAGA(X)5(GalNAc)T(X)5AGAGK
-
 ?
2.4.1.41 UDP-GalNAc + GAGA(X)nT(X)nAGAGK library of unmodified peptides, with randomized residue at position X and n=3 or n=5 Drosophila melanogaster GAGA(X)n(GalNAc)T(X)nAGAGK + UDP
-
 ?
2.4.1.41 UDP-GalNAc + GAGA(X)nT(X)nAGAGK library of unmodified peptides, with randomized residue at position X and n=3 or n=5, random peptide preferences are not significantly altered by increased peptide substrate length n=5 compared to n=3 or the proximity of flanking Gly and Ala residues to the site of glycosylation, major peptide substrate preference determinants are the 2 to 3 residues flanking the site of glycosylation Bos taurus GAGA(X)n(GalNAc)T(X)nAGAGK + UDP
-
 ?
2.4.1.41 UDP-GalNAc + GAGA(X)nT(X)nAGAGK library of unmodified peptides, with randomized residue at position X and n=3 or n=5, random peptide preferences are not significantly altered by increased peptide substrate length n=5 compared to n=3 or the proximity of flanking Gly and Ala residues to the site of glycosylation, major peptide substrate preference determinants are the 2 to 3 residues flanking the site of glycosylation Homo sapiens GAGA(X)n(GalNAc)T(X)nAGAGK + UDP
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide mucin-type O-linked glycosylation of serine or threonine residues Bos taurus UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide mucin-type O-linked glycosylation of serine or threonine residues Homo sapiens UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide mucin-type O-linked glycosylation of serine or threonine residues Drosophila melanogaster UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.41 PGANT
-
 Drosophila melanogaster
2.4.1.41 UDP-GalNAc:polypeptide alpha-N-acetylgalactosaminyltransferase
-
 Drosophila melanogaster
2.4.1.41 UDP-GalNAc:polypeptide alpha-N-acetylgalactosaminyltransferase abbreviated as ppGalNAc T Bos taurus
2.4.1.41 UDP-GalNAc:polypeptide alpha-N-acetylgalactosaminyltransferase abbreviated as ppGalNAc T Homo sapiens